FITC functionalized magnetic core-shell Fe3O4/Ag hybrid nanoparticle for selective determination of molecular biothiols

SENSORS AND ACTUATORS B-CHEMICAL

卷:193

Article

Chen, Ling^1,5  Li, Jinhua¹  Wang, Shasha^1,5  Lu, Wenhui¹  Wu, Aiguo^2,3  Choo, Jaebum⁴  Chen, Lingxin¹ 

[1] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China

[2] Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Key Lab Magnet Mat & Devices, Ningbo 315201, Peoples R China

[3] Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Div Funct Mat & Nanodevices, Ningbo 315201, Peoples R China

[4] Chinese Acad Sci, Dept Bionano Technol, Ningbo 315201, Peoples R China

[5] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

关键词: Molecular biothiols;    Chromogenic detection;    Silver coated magnetic nanoparticles;    Fluorescein isothiocyanate;    Living cells;   

DOI  :  10.1016/j.snb.2013.12.053

来源: Elsevier

【 摘 要 】

A sensing strategy for chromogenic detection of molecular biothiols has been proposed based on fluorescein isothiocyanate (FITC) functionalized magnetic core-shell Fe3O4/Ag hybrid nanoparticles. Ag coated magnetic Fe3O4 nanoparticles were initially synthetized. FITC was subsequently conjugated on the surface of core-shell nanoparticles by Ag-SCN linkage and then the fluorescence of FITC was quenched. Upon addition of molecular biothiols, since the Ag S bond is stronger than Ag-SCN, a place-displacement between thiols and FITC would occur and thereby the fluorescence of FITC would recover. Thus, a fluorescence off-on probe was attained by virtue of biothiols, so after magnetic separation, the fluorescence signal change of FITC in clear solution could be employed for quantitative determination of typical molecular biothiols such as glutathione (GSH) and cysteine (Cys). High sensitivity was obtained with the detection limits of 10 nM and 20 nM for GSH and Cys, respectively. As well as, the assay strategy presented excellent selectivity toward molecular biothiols against other amino acids. Furthermore, confocal imaging was achieved in living cells, indicating that this fluorescent probe is potentially applicable for imaging molecular thiols in biological systems. (C) 2013 Elsevier B.V. All rights reserved.

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