Organic distributed feedback laser for label-free biosensing of ErbB2 protein biomarker

SENSORS AND ACTUATORS B-CHEMICAL

卷:223

Article

Retolaza, Aritz^1,2  Martinez-Perdiguero, Josu^1,2  Merino, Santos^1,2  Morales-Vidal, Marta³  Boj, Pedro G.⁴  Quintana, Jose A.⁴  Villalvilla, Jose M.³  Diaz-Garcia, Maria A.³ 

[1] IK4 Tekniker, Micro & Nano Fabricat Unit, Eibar 20600, Spain

[2] Goiru Kalea 9 Polo Innovac Garaia, MicroGUNE, Arrasate Mondragon 20500, Spain

[3] Univ Alicante, Dept Fis Aplicada, Inst Univ Mat Alicante & Unidad Asociada UA CSIC, E-03080 Alicante, Spain

[4] Univ Alicante, Dept Opt, Inst Univ Mat Alicante & Unidad Asociada UA CSIC, E-03080 Alicante, Spain

关键词: Label-free biosensors;    Organic lasers;    Distributed feedback;    Perylenediimides;    ErbB2 biomarker;   

DOI  :  10.1016/j.snb.2015.09.093

来源: Elsevier

【 摘 要 】

The human epidermal growth factor receptor 2 (ErbB2) protein plays an important role in human malignancies. Its overexpression has been recognized as a feature of a malignant cancerous phenotype in breast cancer cell lines, and has become one of the most widely investigated clinical indicators of breast, ovarian, gastrointestinal and lung cancers. In this work a vertically emitting organic distributed feedback (DFB) laser has been used to detect the ErbB2 protein. This DFB laser consists of a polystyrene (PS) film containing a perylenediimide laser dye, deposited over a second-order one dimensional grating fabricated on fused silica by thermal-nanoimprint lithography and subsequent reactive ion etching processes. Specificity of the system to ErbB2 protein biomarker, achieved by functionalizing the PS with anti-ErbB2 monoclonal antibodies, is demonstrated. A concentration limit of detection for ErbB2 protein of 14 ng/mL has been obtained, and no cross-reactivity has been observed with bovine serum albumin (BSA) and tumor necrosis factor alpha (TNR alpha) proteins. These findings open the possibility of using this type of biosensors in clinical applications. (C) 2015 Elsevier B.V. All rights reserved.

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