Ex situ integration of multifunctional porous polymer monoliths into thermoplastic microfluidic chips

SENSORS AND ACTUATORS B-CHEMICAL

卷:202

Article

Kendall, Eric L.¹  Wienhold, Erik²  Rahmanian, Omid D.³  DeVoe, Don L.^1,3 

[1] Dept Mech Engn, College Pk, MD 20742 USA

[2] Dept Mat Sci Engn, College Pk, MD 20742 USA

[3] Univ Maryland, Dept Bioengn, College Pk, MD 20742 USA

关键词: Thermoplastic;    Microfluidics;    Polymer monolith;    Biosensor;    Immunosensor;   

DOI  :  10.1016/j.snb.2014.06.023

来源: Elsevier

【 摘 要 】

A unique method for incorporating functional porous polymer monolith elements into thermoplastic microfluidic chips is described. Monolith elements are formed in a microfabricated mold, rather than within the microchannels, and chemically functionalized off chip before insertion into solvent-softened thermoplastic microchannels during chip assembly. Because monoliths may be trimmed prior to final placement, control of their size, shape, and uniformity is greatly improved over in situ photopolymerization methods. A characteristic trapezoidal profile facilitates rapid insertion and enables complete mechanical anchoring of the monolith periphery, eliminating the need for chemical attachment to the microchannel walls. Off-chip processing allows the parallel preparation of monoliths of differing compositions and surface chemistries in large batches. Multifunctional flow-through arrays of multiple monolith elements are demonstrated using this approach through the creation of a fluorescent immunosensor with integrated controls, and a microfluidic bubble separator comprising a combination of integrated hydrophobic and hydrophilic monolith elements. (C) 2014 Elsevier B.V. All rights reserved.

【 授权许可】

Free