A portable chemical toxicity biochip based on electronic enzymatic monitoring of cytotoxic effects on fish cells

SENSORS AND ACTUATORS B-CHEMICAL

卷:243

Article

Hara, Tony O.¹  Seddon, Brian²  McClean, Siobhan³  Dempsey, Eithne^1,2 

[1] Ctr Res Electroanalyt Technol CREATE, Dept Sci, Inst Technol, Dublin 24, Ireland

[2] Ctr Appl Sci Hlth, Inst Technol, Microsensors Clin Res & Anal, MiCRA Biodiagnost, Dublin 24, Ireland

[3] CMHI, Inst Technol Tallaght, Dept Sci, Dublin 24, Ireland

关键词: Electrochemical assay;    Cytotoxicity;    Fish cells;    Biosensor;   

DOI  :  10.1016/j.snb.2016.11.097

来源: Elsevier

【 摘 要 】

Herein we describe a miniaturised chemical toxicity analyser with capacity to assess water quality without intervention of laboratory equipment. The device, as presented, integrates living cells and electrode-based sensors to monitor cell viability upon exposure to toxic chemicals. The methodology involved cultured fish cells Poeciliopsis lucidia hepatocellular carcinoma (PLHC-1) and Oncorhynchus mykiss rainbow trout gonad (RTG-2) cells, exposed to toxic chemicals (CdCl2 and pentachlorophenol (PCP)) after which the activity of a cellular enzyme, acid phosphatase (AP) was measured. Reduced AP activity was quantified electrochemically, allowing IC50 values (50% reduction in AP activity) to be determined. Fish cells are a relevant model of the toxicity risks posed by contaminated water to aquatic health. Such cells are relatively slow growing and do not require a source of CO2 for incubation, thus ensuring portability for single-use point of site applications. The 24 hour IC50 values determined for CdCl2 in the fish cell lines (16.2 +/- 0.7 and 91 +/- 11 mu M for PLHC and RTG-2, respectively) were in agreement with those found using the MTT assay (16.3 +/- 1.6 and 164 +/- 96 mu M). In the case of PCP, the IC50 value in PHLC cells (127 +/- 22 mu M) suggested enhanced sensitivity towards PCP compared with the MTT assay (IC50>160 mu M). The optimised electronic assay was transferred to a prototype integrated assay cartridge with associated fluidic and transducer elements. This device (TOXOR) monitored changes in the metabolic state of fish cells, realising high-value toxicity information as a potential early warning on-site screening system. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

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