Tyrosinase biosensor based on a glassy carbon electrode modified with multi-walled carbon nanotubes and 1-butyl-3-methylimidazolium chloride within a dihexadecylphosphate film

SENSORS AND ACTUATORS B-CHEMICAL

卷:188

Article

Vicentini, Fernando Campanha¹  Janegitz, Bruno C.^1,2  Brett, Christopher M. A.³  Fatibello-Filho, Orlando¹ 

[1] Univ Fed Sao Carlos, Ctr Ciencias Exatas & Tecnol, Dept Quim, BR-13560970 Sao Carlos, SP, Brazil

[2] Univ Sao Paulo, Inst Fis Sao Carlos, Grp Biofis Mol Sergio Mascarenhas, BR-13566390 Sao Carlos, SP, Brazil

[3] Univ Coimbra, Fac Ciencias & Tecnol, Dept Quim, P-3004535 Coimbra, Portugal

关键词: 1-Butyl-3-methylimidazolium chloride;    Catechol determination;    Dihexadecylphosphate;    Ionic liquid;    Multi-walled carbon nanotubes;    Tyrosinase biosensor;   

DOI  :  10.1016/j.snb.2013.07.109

来源: Elsevier

【 摘 要 】

A glassy carbon electrode modified with functionalized multi-walled carbon nanotubes (MWCNT), 1-butyl-3-methylimidazolium chloride (ionic liquid = IL) and tyrosinase (Tyr) within a dihexadecylphosphate (DHP) film for the development of a biosensor is proposed. MWCNT, IL and Tyr were efficiently immobilized in the film using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) as crosslinking agents, and which was characterized by cyclic voltammetry (CV) in the presence of catechol. The IL-MWCNT nanocomposite showed good conductivity and biocompatibility with Tyr enzyme, since the biosensor presented biocatalytic activity toward the oxidation of catechol to o-quinone which was electrochemically reduced to catechol at a potential of 0.04 V. The analytical curve ranged from 4.9 x 10(-6) to 1.1 x 10(-3) mol L-1 with a detection limit of 5.8 x 10(-7) mol L-1. The developed Tyr-IL-MWCNT-DHP/GCE biosensor showed a wide linear range, good reproducibility, sensitivity and stability and the biosensor was successfully applied to the determination of catechol in natural water samples, with satisfactory results compared with a spectrophotometric method, at the 95% confidence level. (C) 2013 Elsevier B.V. All rights reserved.

【 授权许可】

Free