Mercury-free and modification-free electroanalytical approach towards bromazepam and alprazolam sensing: A facile and efficient assay for their quantification in pharmaceuticals using boron-doped diamond electrodes

SENSORS AND ACTUATORS B-CHEMICAL

卷:245

Article

Samiec, Petr¹  Svorc, Lubomir²  Stankovic, Dalibor M.^3,4  Vojs, Marian⁵  Marton, Marian⁵  Navratilova, Zuzana¹ 

[1] Univ Ostrava, Fac Sci, Dept Chem, 30 Dubna 22, CZ-70103 Ostrava, Czech Republic

[2] Slovak Univ Technol Bratislava, Fac Chem & Food Technol, Inst Analyt Chem, Radlinskeho 9, SK-81237 Bratislava, Slovakia

[3] Univ Belgrade, Fac Chem, Innovat Ctr, Dept Analyt Chem, Studentski Trg 12-16, Belgrade 11000, Serbia

[4] Univ Belgrade, Inst Nucl Sci Vinca, POB 522, Belgrade 11000, Serbia

[5] Slovak Univ Technol Bratislava, Fac Elect Engn & Informat Technol, Inst Elect & Photon, Ilkovicova 3, SK-81219 Bratislava, Slovakia

关键词: Benzodiazepines;    Boron-doped diamond electrode;    Electrochemical sensor;    Differential pulse voltammetry;    Pharmaceutical tablet;   

DOI  :  10.1016/j.snb.2017.02.023

来源: Elsevier

【 摘 要 】

The boron-doped diamond electrodes (BDDEs) with different dopant levels were applied as advanced, mercury-free and modification-free electroanalytical platforms for simple and reliable quantification of benzodiazepines, bromazepam (BZ) and alprazolam (ALZ). Cyclic voltammetric measurements revealed that the electrode reactions of both analytes were manifested by one irreversible and diffusion-controlled reduction peak at -1.10V in Britton-Robinson buffer of pH 11 for BZ using lab-made BDDE with 1000 ppm B/C ratio and -0.84V in BR buffer of pH 5 for ALZ using commercial BDDE with 1000 ppm B/C ratio. Differential pulse voltammetry was used for construction of calibration curves for BZ and ALZ, respectively, with the analytical parameters as follows: the linear dynamic ranges of 1 x 10(-6)-1 x 10(-4) and 8 x 10(-7)-1 x 10(-4) mol/L, the detection limit of 3.1 x 10(-7) and 6.4 x 10(-7) mol/L and the excellent repeatability with the relative standard deviation below 3% for both drugs. The developed methods were applied to analysis of the pharmaceutical tablets with the recoveries from 97.33 to 100.85% for BZ and from 94.14 to 101.32% for ALZ. The usage of BDDEs as advanced electrochemical sensors in drug analysis represents a facile and effective analytical approach which may replace mercury-based sensors and chemically modified electrodes in previous benzodiazepines sensing. (C) 2017 Elsevier B.V. All rights reserved.

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