Orientation and capturing of antibody affinity ligands: Applications to surface plasmon resonance biochips

SENSORS AND ACTUATORS B-CHEMICAL

卷:158

Article

Bergstrom, Gunnar¹  Mandenius, Carl-Fredrik¹ 

[1] Linkoping Univ, Div Biotechnol, IFM, S-58183 Linkoping, Sweden

关键词: Biosensor;    Affinity interaction;    SPR;    Biacore;    Protein G;    Sensor chip;   

DOI  :  10.1016/j.snb.2011.06.017

来源: Elsevier

【 摘 要 】

A surface plasmon resonance (SPR) sensor chip with immobilized protein G was used for simultaneously capturing, purifying and orienting antibody ligands. The ligands were further stabilized by chemical cross-linking. This procedure of designing the sensor chip improved efficient use of the ligands and could prolong the analytical use. The procedure was evaluated on standard dextran-coated sensor chips onto which commercial semi-purified antibodies towards human serum albumin and human troponin where captured and used for analysing their antigens. The procedure demonstrates a general design approach for presenting the biorecognition element on a biosensor surface which enhances sensitivity, stability and selectivity at the same time as an impure ligand is purified. (C) 2011 Elsevier B.V. All rights reserved.

【 授权许可】

Free