A novel rhodamine-based colorimetric and fluorescent sensor for Hg2+ in water matrix and living cell

SENSORS AND ACTUATORS B-CHEMICAL

卷:232

Article

Hong, Miaomiao^1,2  Lu, Xiangyu^1,2  Chen, Yuhua³  Xu, Dongmei^1,2 

[1] Soochow Univ, Coll Chem Chem Engn & Mat Sci, Suzhou 215123, Jiangsu, Peoples R China

[2] Soochow Univ, Jiangsu Key Lab Adv Funct Polymer Design & Applic, Suzhou 215123, Jiangsu, Peoples R China

[3] Soochow Univ, Coll Preclin Med & Biol Sci, Suzhou 215123, Jiangsu, Peoples R China

关键词: Rhodamine;    Colorimetric sensor;    Fluorescent sensor;    Solid support sensor;    Hg2+;    Cell imaging;   

DOI  :  10.1016/j.snb.2016.03.125

来源: Elsevier

【 摘 要 】

A novel rhodamine-based colorimetric and fluorescent sensor for Hg2+ was reported. In CH3CN/H2O (1/99, v/v), the sensor showed high selectivity and sensitivity to Hg2+ by an emerging absorption peak at 565 nm and a 32-fold fluorescence enhancement at 586 nm with vivid color changes. The absorbance and fluorescence maxima increased linearly with the concentration of Hg2+ in the range of 0-90 mu M. The colorimetric and fluorescent detection limits were 6.36 mu M and 60.78 nM respectively. The sensor could work in a nearly neutral pH span of 6.01-8.57 and exhibited excellent interference immunity and low cytotoxicity. A 1:2 sensor-Hg2+ complex formed with a binding constant of 2.89 x 10(8) M-2. A new sensing mechanism was proposed. The sensor was successfully applied in real sample assay and living cell imaging. Furthermore, the sensor could be supported in low cost cellulose discs for signaling Hg2+ in 100% aqueous solution. (C) 2016 Elsevier B.V. All rights reserved.

【 授权许可】

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