Comparative study of prostate cancer biophysical and migratory characteristics via iterative mechanoelectrical properties (iMEP) and standard migration assays

SENSORS AND ACTUATORS B-CHEMICAL

卷:321

Article

Ghassemi, Parham¹  Harris, Koran S.²  Ren, Xiang¹  Foster, Brittni M.²  Langefeld, Carl D.³  Kerr, Bethany A.²  Agah, Masoud¹ 

[1] Virginia Tech, Bradley Dept Elect & Comp Engn, Blacksburg, VA 24061 USA

[2] Wake Forest Sch Med, Dept Canc Biol, Winston Salem, NC 27157 USA

[3] Wake Forest Sch Med, Dept Biostat & Data Sci, Div Publ Hlth Sci, Winston Salem, NC 27157 USA

关键词: Cell deformability;    Microfluidics;    Impedance;    Cancer;    Migration assays;   

DOI  :  10.1016/j.snb.2020.128522

来源: Elsevier

【 摘 要 】

This study reveals a new microfluidic biosensor consisting of a multi-constriction microfluidic device with embedded electrodes for measuring the biophysical attributes of single cells. The biosensing platform called the iterative mechano-electrical properties (iMEP) analyzer captures electronic records of biomechanical and bioelectrical properties of cells. The iMEP assay is used in conjunction with standard migration assays, such as chemotaxis-based Boyden chamber and scratch wound healing assays, to evaluate the migratory behavior and biophysical properties of prostate cancer cells. The three cell lines evaluated in the study each represent a stage in the standard progression of prostate cancer, while the fourth cell line serves as a normal/healthy counterpart. Neither the scratch assay nor the chemotaxis assay could fully differentiate the four cell lines. Furthermore, there was not a direct correlation between wound healing rate or the migratory rate with the cells' metastatic potential. However, the iMEP assay, through its multiparametric dataset, could distinguish between all four cell line populations with p-value < 0.05. Further studies are needed to determine if iMEP signatures can be used for a wider range of human cells to assess the tumorigenicity of a cell population or the metastatic potential of cancer cells.

【 授权许可】

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