Optimisation of analyte transport in integrated microfluidic affinity sensors for the quantification of low levels of analyte

SENSORS AND ACTUATORS B-CHEMICAL

卷:131

Article

Friedrich, Daniel¹  Please, Colin²  Melvin, Tracy¹ 

[1] Univ Southampton, Optoelect Res Ctr, Highfield SO17 1BJ, England

[2] Univ Southampton, Sch Math, Highfield SO17 1BJ, England

关键词: affinity sensor;    microfluidics;    mass transfer;    laminar flow;    biosensor;    fluidic channel;    detection;    design optimisation;    recognition molecule;    analyte depletion;   

DOI  :  10.1016/j.snb.2007.11.034

来源: Elsevier

【 摘 要 】

New designs for microfluidic channels to be integrated with small-scale affinity sensors for analytical applications are provided. Theoretical approaches demonstrate efficient and uniform mass transfer of the analyte from the bulk flow to small-scale affinity sensors in the base of fluidic channels by (i) active control of the analyte flow speed over the affinity sensor, (ii) non-rectangular channel geometries and (iii) non-uniform distributions of recognition binding sites over the active area of the sensor. The methodology reported provides generic strategies that can be exploited for small-scale sensors in single or multiplex formats. (c) 2007 Elsevier B.V. All rights reserved.

【 授权许可】

Free