New electrochemical approach for the measurement of oxidative DNA damage: Voltammetric determination of 8-oxoguanine at screen-printed graphite electrodes

SENSORS AND ACTUATORS B-CHEMICAL

卷:247

Article

Bernalte, Elena^1,2  Carroll, Michael¹  Banks, Craig E.¹ 

[1] Manchester Metropolitan Univ, Fac Sci & Engn, Manchester M1 5GD, Lancs, England

[2] Univ Extremadura, Fac Ciencias, Dept Quim Analit & IACYS, Avda Elvas S-N, Badajoz 06006, Spain

关键词: Screen;    printed electrodes;    8-Oxoguanine;    DNA oxidation;    Voltammetrya;   

DOI  :  10.1016/j.snb.2017.03.046

来源: Elsevier

【 摘 要 】

Simplification and miniaturisation of analytical methods for the direct detection of DNA damage is a challenging area of research and screen-printed electrodes are a promising alternative approach to analytically/electroanalytically monitor the species involved. In this work we demonstrate that screen-printed graphite macroelectrodes (SPEs) provide useful electrochemical signatures to study the behaviour of 8-oxoguanine (8-oxoGua), which is the most frequent and important marker of oxidative DNA damage and it is widely considered as a biomarker, via differential pulse voltammetry (DPV). Under the optimum experimental conditions, the proposed electrochemical sensing protocol towards 8-oxoGua using SPEs is demonstrated to be possible over the concentration range of 0.1-12 mu M. The response of the SPEs is superior over routinely utilised glassy carbon electrodes in terms of sensitivity with a limit of detection (3 sigma) found to correspond to 0.33 mu M. Reproducibility and repeatability of the proposed methodology at low and high concentrations were also demonstrated. Quantification of 8-oxoGua in the presence of other nucleobases and different compounds of interest which are present in biological fluids was successfully accomplished. Furthermore, proof-of-concept demonstrating the potential use of the developed SPE based methodology for the detection of 8-oxoGua in real complex samples as demonstrated in simulated biological samples (human semen). (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

Free