Continuous-Flow ATP amplification system on a chip

SENSORS AND ACTUATORS B-CHEMICAL

卷:142

Article

Satoh, Tetsuya^1,2  Shinoda, Yasuharu¹  Tokonami, Shiho^1,2  Hirota, Ryuichi¹  Noda, Kenichi^1,2  Kuroda, Akio^1,2  Murakami, Yuji^1,2 

[1] Hiroshima Univ, Grad Sch Adv Sci Matter, Dept Mol Biotechnol, Hiroshima 7398530, Japan

[2] Hiroshima Univ, Res Inst Nanodevice & Bio Syst, Hiroshima 7398530, Japan

关键词: Bioluminescence assay;    ATP amplification;    Continuous-flow system;    PDMS chip;   

DOI  :  10.1016/j.snb.2009.08.020

来源: Elsevier

【 摘 要 】

In this study, we constructed a novel microfluidic device for continuous-flow ATP amplification, using the SU-8:PDMS method. Sepharose beads immobilized with adenylate kinase and pyruvate kinase was packed into a microfluidic chamber to form lamination layer. Dry film type SU-8 was suitable to form a very thick mold for beads column reactor and its dam structure. A good correlation between amplified luminescence and initial ATP concentration was observed in this system. The gradient of amplification when performing six cycles of continuous-flow ATP amplification was 1.72(N). (c) 2009 Elsevier B.V. All rights reserved.

【 授权许可】

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