Rapid and sensitive detection of norovirus antibodies in human serum with a biolayer interferometry biosensor

SENSORS AND ACTUATORS B-CHEMICAL

卷:221

Article

Auer, Sanna¹  Koho, Tiia¹  Uusi-Kerttula, Hanni³  Vesikari, Timo^4,5  Blazevic, Vesna⁵  Hytonen, Vesa P.^2,6 

[1] Univ Tampere, BioMediTech, FIN-33101 Tampere, Finland

[2] Univ Tampere, BioMediTech, Prot Dynam Res Grp, FIN-33101 Tampere, Finland

[3] Univ Tampere, Sch Med, FIN-33101 Tampere, Finland

[4] Univ Tampere, Sch Med, Virol, FIN-33101 Tampere, Finland

[5] Univ Tampere, Sch Med, Vaccine Res Ctr, FIN-33101 Tampere, Finland

[6] Fimlab Labs Ltd, Tampere, Finland

关键词: Biolayer interferometry;    Norovirus;    Virus-like particles (VLPs);    P-particles;    Non-labelled detection;    Fast diagnostics;   

DOI  :  10.1016/j.snb.2015.06.088

来源: Elsevier

【 摘 要 】

Here, we describe the use of a biolayer interferometry biosensor for the fast and sensitive detection of virus-specific antibodies from human serum samples. Norovirus-like particles and norovirus P-particles were used to functionalise the biosensor tip. The detection of antibodies directly from serum samples was challenging, but the addition of a metal chelator (DAB) combined with an anti-human horseradish peroxidase-tagged antibody enabled enhanced detection of virus-specific antibodies in serum dilutions up to 1:100,000. Biolayer interferometry provides results faster than an ELISA, with results in as little as 10-20 min when using pre-functionalised sensors. Therefore, biolayer interferometry combined with DAB enhancement offers an attractive method for quick and sensitive quantification of biomolecules from complicated sample matrices. (C) 2015 Elsevier B.V. All rights reserved.

【 授权许可】

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