A simple and compact smartphone-based device for the quantitative readout of colloidal gold lateral flow immunoassay strips

SENSORS AND ACTUATORS B-CHEMICAL

卷:266

Article

Xiao, Wei¹  Huang, Caihong¹  Xu, Fei¹  Yan, Junjie¹  Bian, Hongfen¹  Fu, Qiangqiang¹  Xie, Kaixin¹  Wang, Lei¹  Tang, Yong^1,2 

[1] Jinan Univ, Guangdong Prov Engn Res Ctr Antibody Drug & Immun, Dept Bioengn, Guangzhou 510632, Guangdong, Peoples R China

[2] Jinan Univ, Inst Food Safety & Nutr, Guangzhou 510632, Guangdong, Peoples R China

关键词: Smartphone;    Ambient light sensor;    Lateral flow immunoassay strip;    Colloidal gold;    Quantitative detection;   

DOI  :  10.1016/j.snb.2018.03.110

来源: Elsevier

【 摘 要 】

Colloidal gold lateral flow immunoassay strips (AuNPs-LFIS) have been widely applied as qualitative diagnostic tools for point-of-care tests (POCT). If strip readers were incorporated, their use could be extended to quantitative analysis. However, their cost and non-portability render commercial strip readers unavailable for use in either home testing, community or rural hospital diagnosis. This is particularly true for on-site testing. Here, a smartphone-based reader was designed and 3D-printed for quantitatively assess AuNPs-LFIS. The basic principle of the devise was relying on a smartphone's ambient light sensor (SPALS). This sensor was harnessed to measure the transmitted light intensities originating from the T-lines on the strips, the transmitted light intensities vary with concentration of AuNP on the T-lines. To validate this approach, our newly developed smartphone's ambient light sensor-based reader (SPALS-reader) was used to readout AuNPs-LFIS of three analytical targets: cadmium ion (Cd2+; limit of detection (LOD) was 0.16 ng/mL), clenbuterol (CL; LOD was 0.046 ng/mL), and porcine epidemic diarrhea virus (PEDV; LOD was 0.055 mu g/mL). The result showed good consistency with the results of conventional image analysis approaches, indicating that the smartphone-based device is appropriate for use in AuNPs-LFIS readouts. Compared with the traditional analysis method, the developed AuNPs-LFIS reader is easier operated, lower cost and more portable, which provided an on-site quantitative analysis tool for AuNPs-LFIS and enhances the applied range of AuNPs-LFIS. (C) 2018 Elsevier B.V. All rights reserved.

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