Highly sensitive fluorogenic sensing of L-Cysteine in live cells using gelatin-stabilized gold nanoparticles decorated graphene nanosheets

SENSORS AND ACTUATORS B-CHEMICAL

卷:259

Article

Thirumalraj, Balamurugan^1,2  Dhenadhayalan, Namasivayam^3,4  Chen, Shen-Ming¹  Liu, Yan-Jin⁵  Chen, Tse-Wei¹  Liang, Po-Huang^5,6  Lin, King-Chuen^3,4 

[1] Natl Taipei Univ Technol, Dept Chem Engn & Biotechnol, Taipei 106, Taiwan

[2] Natl Taiwan Univ Sci & Technol, Dept Chem Engn, Taipei 10607, Taiwan

[3] Natl Taiwan Univ, Dept Chem, Taipei 106, Taiwan

[4] Acad Sinica, Inst Atom & Mol Sci, Taipei 106, Taiwan

[5] Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan

[6] Natl Taiwan Univ, Inst Biochem Sci, Taipei 10617, Taiwan

关键词: Reduced graphene oxide;    Gold nanoparticles;    L Cysteine;    Bioimaging;    Energy transfer;    Fluorescence;   

DOI  :  10.1016/j.snb.2017.12.028

来源: Elsevier

【 摘 要 】

A highly sensitive and selective fluorogenic sensing of L-Cysteine (L-Cys) was implemented based on gelatin stabilized gold nanoparticles decorated reduced graphene oxide (rGO/Au) nanohybrid. Ther GO/Au nanohybrid was prepared by the one-pot hydrothermal method and well characterized by different physiochemical techniques. The nanohybrid exhibits a weak fluorescence of rGO due to the energy transfer from the rGO to Au NPs. The rGO/Au nanohybrid shows enhanced fluorescence activity due to the restoration of quenched fluorescence of rGO/Au nanohybrid in presence of L-Cys. The rGO/Au nanohybrid exhibits much lower detection limit of 0.51 nM for L-Cys with higher selectivity. The fluorescence sensing mechanism arose from the fluorescence recovery due to the stronger interaction between Au NPs and L-Cys, and consequently, the energy transfer was prevented between rGO and Au NPs. The practicability of rGO/Au sensor was implemented by invitro bioimaging measurements in Colo-205 (colorectal adenocarcinoma) and MKN-45 (gastric carcinoma) cancer live cells with excellent biocompatibility. (c) 2017 Elsevier B.V. All rights reserved.

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