Affimer-based impedimetric biosensors for fibroblast growth factor receptor 3 (FGFR3): a novel tool for detection and surveillance of recurrent bladder cancer

SENSORS AND ACTUATORS B-CHEMICAL

卷:326

Article

Thangsunan, Pattanapong^1,7  Lal, Nidhi²  Tiede, Christian^3,4  Moul, Sophie²  Robinson, James, I⁵  Knowles, Margaret A.⁶  Stockley, Peter G.³  Beales, Paul A.²  Tomlinson, Darren C.^3,4  McPherson, Michael J.^3,4  Millner, Paul A.¹ 

[1] Univ Leeds, Fac Biol Sci, Sch Biomed Sci, Leeds LS2 9JT, W Yorkshire, England

[2] Univ Leeds, Fac Engn & Phys Sci, Sch Chem, Leeds, W Yorkshire, England

[3] Univ Leeds, Fac Biol Sci, Sch Mol & Cellular Biol, Leeds, W Yorkshire, England

[4] Univ Leeds, Fac Biol Sci, BioScreening Technol Grp, Leeds, W Yorkshire, England

[5] Univ Leeds, Sch Med, Leeds Inst Cardiovasc & Metab Med, LIGHT Labs, Leeds, W Yorkshire, England

[6] Univ Leeds, Leeds Inst Med Res St Jamess, Leeds, W Yorkshire, England

[7] Natl Inst Metrol, Chem Metrol & Biometry Dept, Bioanal Grp, Klong 5, Klongluang, Pathum Thani, Thailand

关键词: Affimer;    FGFR3;    impedimetric biosensor;    bladder cancer;   

DOI  :  10.1016/j.snb.2020.128829

来源: Elsevier

【 摘 要 】

Fibroblast growth factor receptor 3 (FGFR3) is a transmembrane tyrosine kinase protein in the fibroblast growth factor receptor family, which plays a key role in many biological processes. Over-expression and activating mutations in FGFR3 are frequent in non-invasive bladder cancer, highlighting this protein as a potential biomarker for recurrent bladder cancer detection. Affimer reagents isolated against recombinant FGFR3 were assessed for their affinity using double-sandwich ELISA and SPR. Anti-FGFR3-14 and FGFR3-21 Affimer proteins showed strong binding to FGFR3 and were used for fabrication of impedimetric electrochemical biosensors. A decrease in impedance was observed when the sensors were exposed to increasing concentrations of FGFR3. The successful impedimetric biosensors were capable of detecting sub-pM to nM concentrations of recombinant FGFR3 protein in phosphate buffered saline as well as in synthetic urine.

【 授权许可】

Free