Sensing benzo[a]pyrene-DNA adducts formation via decrease of hybridization reaction

SENSORS AND ACTUATORS B-CHEMICAL

卷:179

Article

Lanzone, V.¹  Scarano, S.²  Del Carlo, M.¹  Corrado, F.³  Esposito, M.³  Minunni, M.²  Compagnone, D.¹ 

[1] Univ Teramo, Dept Food Sci, I-64023 Mosciano Sa, Teramo, Italy

[2] Univ Florence, Dept Chem, I-50019 Florence, Italy

[3] Ist Zooprofilatt Sperimentale Mezzogiorno, I-80055 Naples, Italy

关键词: Benzo[a]pyrene;    DNA sensors;    Surface plasmon resonance;    Electrochemical DNA sensor;    PAHs;   

DOI  :  10.1016/j.snb.2012.10.018

来源: Elsevier

【 摘 要 】

Toxicity of benzo[a]pyrene occurs because of the formation of covalent adducts with DNA guanines. In this work we report the attempt to detect this DNA-adduct using both an electrochemical assay based on gold nanoparticles and a surface plasmon resonance DNA sensor. Detection was achieved via inhibition of the hybridization reaction of oligonucleotide sequences after formation of the covalent adduct. Detecting oligonucleotide sequences have been designed to get high reactivity vs formation of the adduct and benzo[a]-pyrene 7,8-dihydrodiol 9,10-epoxide has been used to generate adducts. Formation of adducts in the nanomolar range was evaluated using the surface plasmon resonance sensor with both the oligonucleotide sequences containing guanines. (C) 2012 Elsevier B.V. All rights reserved.

【 授权许可】

Free