A power compensated differential scanning calorimeter for protein stability characterization

SENSORS AND ACTUATORS B-CHEMICAL

卷:256

Article

Wang, Shuyu¹  Yu, Shifeng²  Siedler, Michael³  Ihnat, Peter M.⁴  Filoti, Dana I.⁴  Lu, Ming⁵  Zuo, Lei² 

[1] SUNY Stony Brook, Dept Mech Engn, Stony Brook, NY 11794 USA

[2] Virginia Tech, Dept Mech Engn, Blacksburg, VA 24061 USA

[3] AbbVie, D-67061 Ludwigshafen, Germany

[4] AbbVie Biores Ctr, Worcester, MA 01605 USA

[5] Brookhaven Natl Lab, Ctr Funct Nanomat, Upton, NY 11973 USA

关键词: Microcalorimeter;    Protein stability;    Differential scanning calorimeter;   

DOI  :  10.1016/j.snb.2017.10.034

来源: Elsevier

【 摘 要 】

This paper presented a power compensated MEMS differential scanning calorimeter (DSC) for protein stability characterization. In this microfabricated sensor, PDMS (Polydimethylsiloxane) and polyimide were used to construct the adiabatic chamber (1 mu L) and temperature sensitive vanadium oxide was used as the thermistor material. A power compensation system was implemented to maintain the sample and reference at the same temperature. The resolution study and step response characterization indicated the high sensitivity (6 V/W) and low noise level (60 mu k) of the device. The test with IgG1 antibody (mAb1) samples showed clear phase transitions and the data was confirmed to be reasonable by comparing it with the results of commercial DSC's test. This device used similar to 1uL sample amount and could complete the scanning process in 4 min, significantly increasing the throughput of the bimolecular thermodynamics study like drug formulation process. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

Free