Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices

SENSORS AND ACTUATORS B-CHEMICAL

卷:255

Article

Rizzo, Riccardo¹  Alvaro, Maria^2,3  Danz, Norbert⁴  Napione, Lucia^2,3  Descrovi, Emiliano¹  Schmieder, Stefan⁵  Sinibaldi, Alberto⁶  Chandrawati, Rona^7,11  Rana, Subinoy⁷  Munzert, Peter⁴  Schubert, Thomas⁸  Maillart, Emmanuel⁹  Anopchenko, Aleksei^6,12  Rivolo, Paola¹  Mascioletti, Alessandro¹⁰  Sonntag, Frank⁵  Stevens, Molly M.⁷  Bussolino, Federico^2,3  Michelotti, Francesco⁶ 

[1] Politecn Torino, Dept Appl Sci & Technol, Cso Duca Abruzzi 24, I-10129 Turin, Italy

[2] IRCCS, Candiolo Canc Inst, Lab Vasc Oncol, Candiolo, Italy

[3] Univ Torino, Dept Oncol, Candiolo, Italy

[4] Fraunhofer Inst Appl Opt & Precis Engn I0F, Albert Einstein Str 7, D-07745 Jena, Germany

[5] Fraunhofer Inst Mat & Beam Technol IWS Dresden, D-01277 Dresden, Germany

[6] Sapienza Univ Rome, Dept Basic & Appl Sci Engn, Via A Scarpa 16, I-00161 Rome, Italy

[7] Imperial Coll London, Dept Mat, London SW7 2AZ, England

[8] KDS Raderberger GmbH, D-01900 Grosshansdorf, Germany

[9] HORIBA Sci, Av Vauve CS 45002, F-91120 Palaiseau, France

[10] LABOR Srl, Via G Peroni, I-00131 Rome, Italy

[11] Univ Sydney, Sch Chem & Biomol Engn, Sydney, NSW 2006, Australia

[12] Baylor Univ, Dept Phys, One Bear Pl 97316, Waco, TX 76798 USA

关键词: Biosensor platform;    Bloch surface waves;    Label-free optical sensor;    Enhanced fluorescence;    Tumor biomarker;    Complex matrices;   

DOI  :  10.1016/j.snb.2017.09.018

来源: Elsevier

【 摘 要 】

We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

Free