A robust bioderived wavelength-specific photosensor based on BLUF proteins

SENSORS AND ACTUATORS B-CHEMICAL

卷:310

Article

Tong, Jing^1,2  Zhang, Peng⁵  Zhang, Lei^3,4  Zhang, Dongwei^3,4  Beratan, David N.^5,6,7  Song, Haifeng^3,4  Wang, Yi^3,4  Li, Tie¹ 

[1] Chinese Acad Sci, Sci & Technol Microsyt Lab, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China

[2] UCAS, Beijing 100049, Peoples R China

[3] Beijing Inst Life, Natl Ctr Prot Sci Beijing, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China

[4] NERCPD, Beijing 102206, Peoples R China

[5] Dept Chem, Durham, NC 27708 USA

[6] Duke Univ, Dept Phys, Durham, NC 27708 USA

[7] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA

关键词: Biosensor;    Graphene;    Photodetection;    Photosensitive proteins;    Blue light using flavin;    Charge transfer;   

DOI  :  10.1016/j.snb.2020.127838

来源: Elsevier

【 摘 要 】

Photosensitive proteins are naturally evolved photosensors that often respond to light signals of specific wavelengths. However, their poor stability under ambient conditions hinders their applications in non-biological settings. In this proof-of-principle study, we grafted the blue light using flavin (BLUF) protein reconstructed with flavin adenine dinucleotide (FAD) or roseoflavin (RoF) onto pristine graphene, and achieved selective sensitivity at 450 nm or 500 nm, respectively. We improved the thermal and operational stability substantially via structure-guided cross-linking, achieving 6-month stability under ambient condition and normal operation at temperatures up to 200 degrees C. Furthermore, the device exhibited rare negative photoconductivity behavior. The origins of this negative photoconductivity behavior were elucidated via a combination of experimental and theoretical analysis. In the photoelectric conversion studies, holes from photoexcited flavin migrated to graphene and recombined with electrons. The device allows facile modulation and detection of charge transfer, and provides a versatile platform for future studies of photoinduced charge transfer in biosensors as well as the development of stable wavelength-selective biophotosensors.

【 授权许可】

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