Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

SENSORS AND ACTUATORS B-CHEMICAL

卷:263

Article

Moshksayan, Khashayar¹  Kashaninejad, Navid²  Warkiani, Majid Ebrahimi³  Lock, John G.^4,5  Moghadas, Hajar¹  Firoozabadi, Bahar¹  Saidi, Mohammad Said¹  Nam-Trung Nguyen² 

[1] Sharif Univ Technol, Sch Mech Engn, Tehran 111559567, Iran

[2] Griffith Univ, Queensland Micro & Nanotechnol Ctr, Nathan, Qld 4111, Australia

[3] Univ Technol Sydney, Sch Biomed Engn, Sydney, NSW 2007, Australia

[4] Univ New South Wales, Sch Med Sci, EMBL Australia Node Single Mol Sci, Sydney, NSW 2052, Australia

[5] Univ New South Wales, ARC Ctr Excellence Adv Mol Imaging, Sydney, NSW 2052, Australia

关键词: Microfluidics;    Spheroids;    Tumor microenvironments;    Microwells;    3D cell cultures;   

DOI  :  10.1016/j.snb.2018.01.223

来源: Elsevier

【 摘 要 】

A cell spheroid is a three-dimensional (3D) aggregation of cells. Synthetic, in-vitro spheroids provide similar metabolism, proliferation, and species concentration gradients to those found in-vivo. For instance, cancer cell spheroids have been demonstrated to mimic in-vivo tumor microenvironments, and are thus suitable for in-vitro drug screening. The first part of this paper discusses the latest microfluidic designs for spheroid formation and culture, comparing their strategies and efficacy. The most recent microfluidic techniques for spheroid formation utilize emulsion, microwells, U-shaped microstructures, or digital microfluidics. The engineering aspects underpinning spheroid formation in these microfluidic devices are therefore considered. In the second part of this paper, design considerations for microfluidic spheroid formationchips and microfluidic spheroid culture chips (mu SFCs and mu SCCs) are evaluated with regard to key parameters affecting spheroid formation, including shear stress, spheroid diameter, culture medium delivery and flow rate. This review is intended to benefit the microfluidics community by contributing to improved design and engineering of microfluidic chips capable of forming and/or culturing three-dimensional cell spheroids. (C) 2018 Elsevier B.V. All rights reserved.

【 授权许可】

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