Detecting single-abasic residues within a DNA strand immobilized in a biological nanopore using an integrated CMOS sensor

SENSORS AND ACTUATORS B-CHEMICAL

卷:177

Article

Kim, Jungsuk¹  Maitra, Raj D.¹  Pedrotti, Ken²  Dunbar, William B.¹ 

[1] Univ Calif Santa Cruz, Dept Comp Engn, Santa Cruz, CA 95064 USA

[2] Univ Calif Santa Cruz, Dept Elect Engn, Santa Cruz, CA 95064 USA

关键词: Biomedical instrumentation;    CMOS current-measuring sensor;    DNA-binding proteins;    Gene sequencer;    Nanopore technology;    Potentiostat;    Single-molecule science;   

DOI  :  10.1016/j.snb.2012.11.027

来源: Elsevier

【 摘 要 】

In this paper, we demonstrate the application of a novel current-measuring sensor (CMS) customized for nanopore applications. The low-noise CMS is fabricated in a 0.35 mu m CMOS process and is implemented in experiments involving DNA captured in an a-hemolysin (alpha-HL) nanopore. Specifically, the CMS is used to build a current amplitude map as a function of varying positions of a single-abasic residue within a homopolymer cytosine single-stranded DNA (ssDNA) that is captured and held in the pore. Each ssDNA is immobilized using a biotin-streptavidin linkage. Five different DNA templates are measured and compared: one all-cytosine ssDNA, and four with a single-abasic residue substitution that resides in or near the similar to 1.5 nm aperture of the a-HL channel when the strand is immobilized. The CMOS CMS is shown to resolves the similar to 5 angstrom displacements of the abasic residue within the varying templates. The demonstration represents an advance in application-specific circuitry that is optimized for small-footprint nanopore applications, including genomic sequencing. (c) 2012 Elsevier B.V. All rights reserved.

【 授权许可】

Free