All-fiber all-optical quantitative polymerase chain reaction (qPCR)

SENSORS AND ACTUATORS B-CHEMICAL

卷:323

Article

Li, Xuegang^1,2,3  Nguyen, Linh, V^2,3  Hill, Kelly^4,5  Ebendorff-Heidepriem, Heike^2,3,6  Schartner, Erik P.^2,3,6  Zhao, Yong¹  Zhou, Xue¹  Zhang, Yanan¹  Warren-Smith, Stephen C.^2,3,6 

[1] Northeastern Univ, Coll Informat Sci & Engn, Shenyang 110819, Liaoning, Peoples R China

[2] Univ Adelaide, Inst Photon & Adv Sensing, Adelaide, SA 5005, Australia

[3] Univ Adelaide, Sch Phys Sci, Adelaide, SA 5005, Australia

[4] South Australian Res & Dev Inst, Urrbrae, SA 5064, Australia

[5] Univ Adelaide, Sch Agr Food & Wine, Adelaide, SA 5005, Australia

[6] Univ Adelaide, ARC Ctr Excellence Nanoscale BioPhoton, Adelaide, SA 5005, Australia

关键词: Quantitative polymerase chain reaction;    Optical fiber sensor;    Biosensor;    DNA detection;    Microcavity;    Label-free;   

DOI  :  10.1016/j.snb.2020.128681

来源: Elsevier

【 摘 要 】

Quantitative polymerase chain reaction (qPCR), the real-time amplification and measurement of a targeted DNA molecule, has revolutionized the biological sciences and is routinely applied in areas such as medical diagnostics, forensics, and agriculture. Despite widescale use of qPCR technology in the lab, the availability of low-cost and high-speed portable systems remains one of the barriers to routine in-field implementation. Here we propose and demonstrate a potential solution using a photonics-based qPCR system. By using an all-optical approach, we achieve ultra-fast temperature response with real-time temperature feedback using nanoliter scale reaction volumes. The system uses a microcavity to act as a nanoliter scale reaction vessel with a laser-driven and optically monitored temperature cycling system for ultrafast thermal cycling and incorporates an all-fiber fluorescence excitation/detection system to achieve real-time, high sensitivity fluorescence monitoring of the qPCR process. Further, we demonstrate the potential of the system to operate as a label-free qPCR system through direct optical measurement of the sample refractive index. Due to advantages in portability and fabrication simplicity, we anticipate that this platform technology will offer a new strategy for fundamental techniques in biochemistry applications, such as point-of-care and remote diagnostics.

【 授权许可】

Free