Peptide decorated gold nanoparticle/carbon nanotube electrochemical sensor for ultrasensitive detection of matrix metalloproteinase-7

SENSORS AND ACTUATORS B-CHEMICAL

卷:325

Article

Palomar, Quentin¹  Xu, XingXing¹  Selegard, Robert²  Aili, Daniel²  Zhang, Zhen¹ 

[1] Uppsala Univ, Angstrom Lab, Dept Elect Engn, Div Solid State Elect, SE-75121 Uppsala, Sweden

[2] Linkoping Univ, Dept Phys Chem & Biol IFM, Div Biophys & Bioengn, Mat Mol Lab, SE-58183 Linkoping, Sweden

关键词: Biosensor;    Carbon nanotubes;    Gold nanoparticles;    Peptide;    MMP-7;    Gold electrode;    Differential pulse voltammetry;   

DOI  :  10.1016/j.snb.2020.128789

来源: Elsevier

【 摘 要 】

Matrix Metalloproteinase-7 (MMP-7) is a proteolytic enzyme overexpressed in different pathological conditions, including cancer, infection, and cardiovascular diseases, and is a relevant diagnostic biomarker and potential drug target. Here we demonstrate rapid and selective detection of MMP-7 with a limit-of-detection of 6 pg/mL and a dynamic range from 1 x 10(-2) to 1 x 10(3) ng/mL using a peptide decorated gold nanoparticle/carbon nanotube electrochemical sensor. The sensor could be operated in diluted human serum and synthetic urine, with high specificity towards MMP-7. Moreover, the integration of nanomaterials in the sensing electrode significantly increased the signal-to-background ratio and strongly improved the stability of the sensor when compared to a conventional gold electrode. The simple and cost-effective fabrication and the ease of use make this sensor a very promising protease detection device for diagnostics and drug development.

【 授权许可】

Free