How the surrounding environment affects the biosensing performance of resonant nanopillars arrays: Under dry conditions or immersed in fluid

SENSORS AND ACTUATORS B-CHEMICAL

卷:259

Article

Hernandez, Ana L.¹  Casquel, Rafael^1,2  Holgado, Miguel^1,2  Cornago, Inaki³  Fernandez, Fatima³  Ciaurriz, Paula³  Sanza, Francisco J.⁴  Santamaria, Beatriz¹  Maigler, Maria V.^1,3  Quintero, Sergio¹  Fe Laguna, Maria^1,2 

[1] Univ Politecn Madrid, Ctr Biomed Technol, Campus Montegancedo, Madrid 28223, Spain

[2] Univ Politecn Madrid, Dept Appl Phys, Escuela Super Ingenieros Ind, C Jose Gutierrez Abascal 2, E-28006 Madrid, Spain

[3] Cemitec, ADItech, Plaza Cein 4, Noain 31110, Spain

[4] Univ Politecn Madrid, Bio Opt Detect, Ctr Empresas, Madrid 28223, Spain

关键词: Biosensing enhance between dry/wet conditions;    Optical biosensor;    Resonant nanopillars;    In-situ diagnosis;    Vertical optical interrogation;    Label-free;   

DOI  :  10.1016/j.snb.2017.11.029

来源: Elsevier

【 摘 要 】

In this work we demonstrate the advantage of performing the biosensing process of a refractive index optical biosensor under dry conditions, in comparison with the biosensing structure immersed in fluid. We developed a biosensing experiment over a specific transducer based on resonant nanopillars (R-NPs) arrays. The optical interrogation to monitor the recognition events was firstly performed with the R-NPs in dry, only in contact with the air, and secondly with the R-NPs immersed in water. We observed a significant enhance in the sensitivity of the biosensing curve response for the R-NPs in dry conditions, leading an improvement of the Limit of Detection (LoD) in more than one order of magnitude. These results are also in good correlation with 3D-Finite difference time domain simulations carried out for both fluid conditions. According to this result, any interferometric optical bio-transducer for in-situ diagnosis will improve the sensitivity in case it can operate in dry conditions. Moreover, measuring in simple drops of biological samples, in dry conditions, will be a relevant issue for Point of Care Devices. (c) 2017 Elsevier B.V. All rights reserved.

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