Functionalization of bamboo-like carbon nanotubes with 3-mercaptophenylboronic acid-modified gold nanoparticles for the development of a hybrid glucose enzyme electrochemical biosensor

SENSORS AND ACTUATORS B-CHEMICAL

卷:216

Article

Eguilaz, Marcos¹  Villalonga, Reynaldo²  Pingarron, J. M.²  Ferreyra, Nancy F.¹  Rivas, Gustavo A.¹ 

[1] Univ Nacl Cordoba, Fac Ciencias Quim, Dept Fisicoquim, INFIQC, RA-5000 Cordoba, Argentina

[2] Univ Complutense Madrid, Fac Ciencias Quim, Dept Quim Analit, E-28040 Madrid, Spain

关键词: Bamboo like multiwalled carbon nanotubes;    Carbon nanotubes dispersion;    Polyethylenimine;    Glucose oxidase;    Boronic acid;    Gold nanoparticles;    Amperometric glucose biosensor;   

DOI  :  10.1016/j.snb.2015.03.112

来源: Elsevier

【 摘 要 】

We report for the first time a sensitive and selective glucose biosensor based on the immobilization at glassy carbon electrodes (GCE) of a new hybrid nanomaterial consisting of gold nanoparticles functionalized with 3-mercaptophenyl boronic acid (AuNPs-B(OH)(2)) and bamboo-like multiwall carbon nanotubes (bMWCNT) dispersed in hyperbranched polyethyleneimine (PEI). The presence of boronic acid residues allowed the supramolecular immobilization of glucose oxidase (GOx) as a model glycoenzyme while the hybrid nanomaterial Au(NP)-bMWCNT produces a synergistic effect on the catalytic detection of the enzymatically generated hydrogen peroxide. The resulting functionalized nanomaterials were characterized by TEM, FT-IR and electrochemical techniques. The sensitivity at 0.700 V was (3.26 + 0.03) mA M-1 (28.6 mAM(-1) cm(-2)), with a linear range between 2.50 x 10(-4)M and 5.00 x 10(-3) M, a detection limit of 0.8 p,M and a quantification limit of 2.4 p,M. The biocatalytic layer demonstrated to be highly reproducible with R.S.D. values of 8.6% for 10 successive amperometric calibrations using the same surface, and 4.5% for ten different bioelectrodes. The sensitivity of the biosensor after 14 days of storage at 4 degrees C remained at 86.1% of its original value. The combination of the excellent dispersing properties of PEI, the stability of the bMWCNT-PEI dispersion, the synergistic effect of AuNPs-B(OH)(2) and bMWCNT towards the electrooxidation of hydrogen peroxide, and the robust immobilization of GOx at AuNP-B(OH)(2) allowed building a sensitive, reproducible and stable amperometric biosensor for the quantification of glucose in beverages and milk samples. (C) 2015 Published by Elsevier B.V.

【 授权许可】

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