Application of 4-amino-N-adamantylphthalimide solvatochromic dye for fluorescence microscopy in selective visualization of lipid droplets and mitochondria

SENSORS AND ACTUATORS B-CHEMICAL

卷:286

Article

Bencic, Patricia¹  Mandic, Leo²  Dzeba, Iva³  Bujak, Ivana Tartaro³  Biczok, Laszlo⁴  Mihaljevic, Branka³  Mlinaric-Majerski, Kata²  Weber, Igor⁵  Kralj, Marijeta¹  Basaric, Nikola² 

[1] Rudjer Boskovic Inst, Div Mol Med, Bijenicka Cesta 54, Zagreb 10000, Croatia

[2] Rudjer Boskovic Inst, Dept Organ Chem & Biochem, Zagreb, Croatia

[3] Rudjer Boskovic Inst, Div Mat Chem, Zagreb, Croatia

[4] Hungarian Acad Sci, Res Ctr Nat Sci, Inst Mat & Environm Chem, POB 286, H-1519 Budapest, Hungary

[5] Rudjer Boskovic Inst, Div Mol Biol, Zagreb, Croatia

关键词: Aminophthalimide;    Biological membranes;    Fluorescent dyes;    Confocal fluorescence microscopy;    Nonpolar lipids;   

DOI  :  10.1016/j.snb.2019.01.102

来源: Elsevier

【 摘 要 】

4-Amino-N-adamantylphthalimide (1) is a dye with moderate absorptivity (in CH3CN epsilon(363) = 4200 M-1 cm(-1)) and high quantum yield of fluorescence (Phi(F) = 0.15-0.80) that exhibits fluorosolvatochromic properties. The dye can be excited at 405 nm and the position of fluorescence maximum and the Stokes shift are well correlated with the E-T(30) parameter. The excitation in the near-visible part of the spectrum and low cytotoxicity allow use of the dye in live cell microscopy. Due to its amphiphilic character, the dye stains artificial membranes in lipo-somes. Using confocal microscopy on two human cancer cell lines, we have shown that 1 stains primarily intracellular lipid droplets. Colocalization experiments with different organelle markers indicated that 1 additionally stains mitochondrial membranes. The fluorosolvatochromism of 1 allows the simultaneous visualization of mitochondria and intracellular lipid droplets in two separate emission channels, which has a potential use in cells and tissues exhibiting intense oxidative metabolism of lipids.

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