A colorimetric paper sensor for lactate assay using a cellulose-Binding recombinant enzyme

SENSORS AND ACTUATORS B-CHEMICAL

卷:238

Article

Dai, Guoying^1,2  Hu, Jinglin²  Zhao, Xueyan³  Wang, Ping^1,2 

[1] East China Univ Sci & Technol, Biomed Nanotechnol Ctr, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[2] Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA

[3] Calif Baptist Univ, Dept Chem Engn, Riverside, CA 92504 USA

关键词: Paper-based biosensor;    Cellulose binding domain;    Lactate;    Lactate dehydrogenase;    Enzyme;    Biocatalysis;   

DOI  :  10.1016/j.snb.2016.07.008

来源: Elsevier

【 摘 要 】

A new fabrication technology for effective preparation of paper-based biosensors using affinity-binding enzymes is developed. Lactate dehydrogenase (LDH) fused with a cellulose binding domain (CBD) is produced to allow one-step purification and immobilization of the enzyme on paper. Results demonstrate that the binding capacity of the recombinant enzyme is as high as 22 mg-enzyme/g-paper, with much improved stability (by a factor of 25-fold) in comparison to the native parent enzyme. That ensures the solid phase bioassay reactions on surface of the paper sensor are not limited by enzyme availability, and affords much extended sensor shelf life at the same time. The paper sensor shows a linear detection range of 0.5-8 mM of lactate, with changes in color intensity detectable within a much broader concentration range examined (up to 16 mM). The colorimetric display is suited for readouts using either human eyes or hand-held imaging devices such as smart phones. This work promises of a new strategy of developing high performance paper-based sensors for point-of-care diagnosis applications. (C) 2016 Published by Elsevier B.V.

【 授权许可】

Free