Lateral flow test for visual detection of multiple MicroRNAs

SENSORS AND ACTUATORS B-CHEMICAL

卷:264

Article

Zheng, Wanli¹  Yao, Li¹  Teng, Jun¹  Yan, Chao¹  Qin, Panzhu¹  Liu, Guodong²  Chen, Wei¹ 

[1] Hefei Univ Technol, Sch Food Sci & Engn, Hefei 230009, Anhui, Peoples R China

[2] North Dakota State Univ, Dept Chem & Biochem, Fargo, ND 58105 USA

关键词: Lateral flow biosensor;    microRNA;    Simultaneous detection;    Multiplex detection;    Serum sample;   

DOI  :  10.1016/j.snb.2018.02.159

来源: Elsevier

【 摘 要 】

The authors describe a rapid and low-cost approach for multiplex microRNA(miRNA) assay on lateral flow nucleic acid biosensor (LFNAB). The principle of assay is based on sandwich-type nucleic acid hybridization reactions to produce gold nanoparticle (GNP)-attached complexes (ssDNA-microRNA-ssDNA/GNPs), which are captured and visualized on the test zone of LFNAB. By designing three different test zones on LFNAB, simultaneous detection of microRNA-21, microRNA-155 and microRNA-210 was achieved with an adding-measuring model by using GNP as visual tag. The method was challenged by testing the microRNAs in spiked serum samples with satisfied results. In our perception, the test is a particularly valuable tool for clinical application and biomedical diagnosis, particularly in limited resource settings. (C) 2018 Elsevier B.V. All rights reserved.

【 授权许可】

Free