Reagent- and actuator-free analysis of individual erythrocytes using three-dimensional quantitative phase imaging and capillary microfluidics

SENSORS AND ACTUATORS B-CHEMICAL

卷:348

Article

Ryu, DongHun^1,2  Nam, Hyeono³  Jeon, Jessie S.^2,3  Park, YongKeun^1,2,4 

[1] Korea Adv Inst Sci & Technol KAIST, Dept Phys, Daejeon 34141, South Korea

[2] Korea Adv Inst Sci & Technol, KAIST Inst Hlth Sci & Technol, Daejeon 34141, South Korea

[3] Korea Adv Inst Sci & Technol, Dept Mech Engn, Daejeon 34141, South Korea

[4] Tomocube Inc, Daejeon 34051, South Korea

关键词: Blood test;    Label-free imaging;    Rapid point-of-care test;    Capillary microfluidics;    Quantitative phase imaging;   

DOI  :  10.1016/j.snb.2021.130689

来源: Elsevier

【 摘 要 】

Histopathological examination of blood cells plays a crucial role in the diagnosis of various diseases. However, it involves time-consuming and laborious staining procedures required for microscopic review by medical experts and is not directly applicable for point-of-care diagnosis in resource-limited locations. This study reports a dilution-, actuation-and label-free method for the analysis of individual red blood cells (RBCs) using a capillary microfluidic device and quantitative phase imaging. Blood, without any sample treatment, is directly loaded into a micrometer-thick channel such that it forms a quasi-monolayer inside the channel. The morphological and biochemical properties of RBCs, including hemoglobin concentration, hemoglobin content, and corpuscular volume, were retrieved using the refractive index tomograms of individual RBCs measured using 3D quantitative phase imaging. The deformability of individual RBCs was also obtained by measuring the dynamic membrane fluctuations. The proposed framework applies to other imaging modalities and biomedical applications, facilitating rapid and cost-effective diagnosis and prognosis of diseases.

【 授权许可】

Free