Evanescent-wave cavity ring-down detection of cytochrome c on surface-modified prisms

SENSORS AND ACTUATORS B-CHEMICAL

卷:139

Article

van der Sneppen, L.¹  Gooijer, C.¹  Ubachs, W.¹  Ariese, F.¹ 

[1] Vrije Univ Amsterdam, Ctr Laser, NL-1081 HV Amsterdam, Netherlands

关键词: Surface analysis;    Liquid-phase CRDS;    Surface modification;    Absorption detection;    Laser spectroscopy;   

DOI  :  10.1016/j.snb.2009.03.048

来源: Elsevier

【 摘 要 】

Adsorption kinetics and molecular interactions on different Surface interfaces are studied by means of evanescent-wave cavity ring-down spectroscopy, using total internal reflection surfaces Onto Which different self-assembled monolayers are covalently attached. The adsorption of cytochrome c (a positively charged, spherical heme protein) to a negatively charged bare silica surface, as well as to C-18-coated (hydrophobic) and C3NH2-coated (positively charged) silica have been Studied. It is experimentally verified that these Surface layers do not interfere with the sensitive measurement of adsorbed cyt c monolayers Using the evanescent wave in a ring-down scheme. Attaching monolayers covalently to the silica total internal reflection Surface is a first step towards the development of a biosensor that makes use of immobilized biomolecules for specific detection of analytes in Solution. (C) 2009 Elsevier B.V. All rights reserved.

【 授权许可】

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