A hemin-based molecularly imprinted polymer (MIP) grafted onto a glassy carbon electrode as a selective sensor for 4-aminophenol amperometric

SENSORS AND ACTUATORS B-CHEMICAL

卷:152

Article

Martins Neto, Jose de Ribamar²  Rodrigues Santos, Wilney de Jesus^1,3  Rodrigues Lima, Phabyanno^1,3  Carvalho Neiva Tanaka, Sonia Maria²  Tanaka, Auro Atsushi^2,3  Kubota, Lauro Tatsuo^1,3 

[1] Univ Estadual Campinas, Inst Quim, BR-13084971 Campinas, SP, Brazil

[2] Univ Fed Maranhao, Ctr Ciencias Exatas & Tecnol, BR-65085580 Sao Luis, MA, Brazil

[3] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bionalit, BR-13084971 Campinas, SP, Brazil

关键词: 4-Aminophenol;    Amperometric sensor;    Hemin;    Molecularly imprinted polymer;   

DOI  :  10.1016/j.snb.2010.12.010

来源: Elsevier

【 摘 要 】

Molecular imprinting technology is becoming a versatile tool for the preparation of tailor-made molecular recognition elements. This work investigates the performance of a hemin-modified molecularly imprinted polymer (MIP) used as an amperometric sensor for the detection of 4-aminophenol (4-APh). MIP particles were prepared by the precipitation polymerization method with hemin introduced as the catalytic center to mimic the active site of peroxidase. 4-APh was used as the template molecule, methacrylic acid (MAA) as the functional monomer, trimethylolpropane trimethacrylate (TRIM) as the cross-linker and 2,2'-azobisisobutyronitrile (AIBN) as the initiator. The synthesized polymer particles were characterized in terms of particle size, porosity and morphology. The amperometric sensor used for 4-APh detection was prepared by modifying a glassy carbon electrode surface with the hemin-based MIP. Under optimized operational conditions, a linear response was obtained in the range of 10.0-90.0 mu mol L-1. with a sensitivity of 5.5 nA L mu mol(-1) and a detection limit of 3.0 mu mol L-1. The sensor showed good repeatability (RSD=2.7% for n=7). It exhibited to be very selective for 4-APh even in the presence of structurally similar compounds (2-aminophenol, catechol, guaiachol, 2-cresol and chloroguaiachol). Recoveries in the range 93-111% were obtained using the sensor for the determinations of 4-APh in tap and river water samples. (C) 2010 Elsevier B.V. All rights reserved.

【 授权许可】

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