Sensitive detection of mitragynine from Mitragyna speciosa Korth using an electrochemical immunosensor based on multiwalled carbon nanotubes/ chitosan- modified carbon electrode

SENSORS AND ACTUATORS B-CHEMICAL

卷:345

Article

Mustafa, Radhiahtul Raehan¹  Sukor, Rashidah^1,2  Eissa, Shimaa³  Shahrom, Ainaa Najihah¹  Saari, Nazamid¹  Nor, Siti Mariam Mohd⁴ 

[1] Univ Putra Malaysia, Fac Food Sci & Technol, Dept Food Sci, Serdang 43400, Selangor, Malaysia

[2] Univ Putra Malaysia, Inst Trop Agr & Food Secur, Lab Food Safety & Food Integr, Serdang 43400, Selangor, Malaysia

[3] Alfaisal Univ, Dept Chem, Al Zahrawi St, Riyadh 11533, Saudi Arabia

[4] Univ Putra Malaysia, Fac Sci, Dept Chem, Serdang 43400, Selangor, Malaysia

关键词: Mitragynine;    Opioid;    Multi walled carbon nanotubes;    Chitosan;    Carbon electrode;   

DOI  :  10.1016/j.snb.2021.130356

来源: Elsevier

【 摘 要 】

The indole alkaloid, mitragynine, is the prominent substance in Mitragyna speciosa Korth. (kratom), the use of which has led to addiction potential and adverse health effects. In this study, an electrochemical immunosensor was developed for the first time for the sensitive and rapid detection of mitragynine. Multiwalled carbon nanotubes (MWCNTs)/ chitosan (CS) nanocomposite was utilised as modifier for the sensor fabrication. The detection of mitragynine was based on an indirect competitive assay where, the electrochemical signal was produced from the enzymatic reaction of HRP-modified secondary antibody using 3,3',5,5'-tetramethylbenzidine (TMB) as substrate. The electrochemical immunosensor exhibited higher sensitivity (10-fold) over conventional ELISA with a limit of detection (LOD) of 0.018 mu g/mL and limit of quantification (LOQ) of 0.06 mu g/mL. Detection of mitragynine in spiked urine samples resulted in good recoveries between 70-110 %. Hence, the developed immunosensor is a useful tool to detect mitragynine in urine to control the misuse of kratom.

【 授权许可】

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