Measurement of oocyte temporal maturation process by means of a simple optical micro-system

SENSORS AND ACTUATORS B-CHEMICAL

卷:157

Article

Vidberg, Florie^2,3  Zeggari, Rabah¹  Pieralli, Christian¹  Amiot, Clotilde^2,3  Roux, Christophe^2,3  Wacogne, Bruno¹ 

[1] Fac Sci & Tech, Inst FEMTO ST, CNRS, Dept Opt PM Duffieux,UMR 6174, F-25030 Besancon, France

[2] IFR 133 IBCT Univ Franche Comte, CHU St Jacques, Serv Genet Histol Biol Dev & Reprod, F-25030 Besancon, France

[3] IFR 133 IBCT Univ Franche Comte, CHU St Jacques, Clin Invest Ctr CIC IFR IBCT 133, F-25030 Besancon, France

关键词: In vitro fertilization;    Maturation estimation;    Optical microsystems;    Transmission spectrum measurement;   

DOI  :  10.1016/j.snb.2011.03.021

来源: Elsevier

【 摘 要 】

We present a simple optical micro-system used to measure the transmission spectra of oocytes in order to qualify their maturation stage. Two applications of the device are possible: (i) the evaluation of the maturation stages of oocytes, and (ii) the development of a fertilization indicator. For the first application, CV, MI and MII oocytes were also analysed. Transmission spectra allow the 3 maturation stages to be identified but cannot be used to estimate the maturity of an unknown oocyte. Oocytes are subject to continuous development. This is why spectral separation of the 3 maturation stages cannot be made although they can be visually identified. However, the visual observation remains biologist-dependent. We therefore investigated the temporal maturation evolution of the oocytes in terms of transmission spectra and probability analysis. Results show that oocytes to be fertilized should not only be chosen in the MII stage, but also at the right time during the MII stage. This particular aspect requires further investigation. However, spectral measurements could be used as a technique for monitoring the maturation evolution of the oocytes. Fertilized oocytes exhibiting fertilization abnormalities were also tested. The device proved to be an efficient fertilization indicator. (C) 2011 Elsevier B.V. All rights reserved.

【 授权许可】

Free