Optofluidic label-free SERS platform for rapid bacteria detection in serum

SENSORS AND ACTUATORS B-CHEMICAL

卷:300

Article

Hunter, Robert¹  Sohi, Ali Najafi²  Khatoon, Zohra³  Berthiaume, Vincent R.⁴  Alarcon, Emilio, I^3,5  Godin, Michel^1,2,6  Anis, Hanan⁷ 

[1] Univ Ottawa, Ottawa Carleton Inst Biomed Engn, Ottawa, ON K1N 6N5, Canada

[2] Univ Ottawa, Dept Phys, Ottawa, ON K1N 6N5, Canada

[3] Univ Ottawa, Heart Inst, Cardiac Surg Res, Ottawa, ON K1Y 4W7, Canada

[4] Univ Ottawa, Dept Chem & Biol Engn, Ottawa, ON K1N 6N5, Canada

[5] Univ Ottawa, Fac Med, Dept Biochem Microbiol & Immunol, Ottawa, ON K1N 6N5, Canada

[6] Univ Ottawa, Dept Mech Engn, Ottawa, ON K1N 6N5, Canada

[7] Univ Ottawa, Sch Elect Engn & Comp Sci, Ottawa, ON K1N 6N5, Canada

关键词: Surface enhanced Raman scattering;    Optofluidic;    Label-free detection;    Machine learning;    Hospital acquired infection;   

DOI  :  10.1016/j.snb.2019.126907

来源: Elsevier

【 摘 要 】

The prevalence of hospital acquired infections and antibiotic resistant pathogens necessitates the development of bacteria sensing systems that do not require sample amplification via conventional cell culturing, which can be prohibitively time-consuming. To meet this need, we designed an optofluidic Raman detection platform which utilized a microfluidic driven hollow-core photonic crystal fiber, which in combination with silver nanoparticles, provides a large enhancement to the Raman signal. By confining both light and cells within this fiber, spectral events generated by the flowing cells facilitates a novel method of cell counting to simultaneously quantify and qualify infections. Counting is performed automatically by a genetically optimized support vector machine learning algorithm that was previously developed by our group. The microfluidic system can be regenerated multiple times, and allows for online detection of planktonic bacteria to levels as low as 4 CFU/mL in 15 min. This compares favourably to other methods currently under development such as qPCR and biosensing techniques. Furthermore, Raman spectral differences between bacteria allow for inherent multiplexed detection in serum, by adding another layer to the learning algorithm. Further development of this device has promising potential as a rapid point-of-care system for infection management in the clinic.

【 授权许可】

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