Electroanalytical thread-device for estriol determination using screen-printed carbon electrodes modified with carbon nanotubes

SENSORS AND ACTUATORS B-CHEMICAL

卷:241

Article

Ochiai, Leticia M.¹  Agustini, Deonir¹  Figueiredo-Filho, Luiz C. S.²  Banks, Craig E.³  Humberto Marcolino-Junior, Luiz¹  Bergamini, Marcio F.¹ 

[1] Univ Fed Parana UFPR, Lab Sensores Eletroquim LabSensE, Dept Quim, BR-81531980 Curitiba, Parana, Brazil

[2] Inst Fed Parana, Campus Paranavai, BR-87703536 Paranavai, PR, Brazil

[3] Manchester Metropolitan Univ, Fac Sci & Engn, Chester St, Manchester M1 5GD, Lancs, England

关键词: Microfluidic systems;    Thread-based electroanalytical device;    Estriol determination;    Screen printed electrodes;   

DOI  :  10.1016/j.snb.2016.10.150

来源: Elsevier

【 摘 要 】

Microflow systems are powerful analytical tools that explore similar principles of typical flow injection analysis driven to in a microfluidic device. Generally, microfluidic devices can promote a low consumption of reagents and samples, high speed of analysis and possibility of portability. Several advances have been reached applying a simple and low cost device based on cotton thread as microfluidic channel where the transportation of solutions is based on capillary force helped by gravity. In the present work, we have demonstrated the versatility of thread-based electroanalytical devices (mu TED) constructed using a cotton thread as the solution channel and screen-printed electrodes (SPE) surface modified with carbon nanotubes (CNT) as electrochemical detectors for the amperometric determination of estriol hormone in pharmaceutical samples. The parameters involved in the amperometric detection and microflow system were studied and optimized, using the best experimental conditions (flow rate of 0.50 mu L s(-1), 10 mm of analytical path, 2.0 mu L of volume of injection and potential of detection of 0.75 V) a linear response was observed for concentration range (LDR) of 1.0 to 1000 mu mol L-1 with limits of detection (LOD) and quantification (LOQ) of 0.53 mu molL(-1) and 1.77 mu molL(-1), respectively, and frequency of injection of 32 per hour. The proposed methodology was applied for determination of estriol in commercial samples and results were compared with those provided by spectrophotometric method (official methodology). The obtained results are in agreement at a 95% of confidence level. (C) 2016 Published by Elsevier B.V.

【 授权许可】

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