Long nanoneedle-electrode devices for extracellular and intracellular recording in vivo

SENSORS AND ACTUATORS B-CHEMICAL

卷:258

Article

Kubota, Yoshihiro¹  Yamagiwa, Shota¹  Sawahata, Hirohito¹  Idogawa, Shinnosuke¹  Tsuruhara, Shuhei¹  Numano, Rika^2,3  Koida, Kowa^3,4  Ishida, Makoto^1,3  Kawano, Takeshi¹ 

[1] Toyohashi Univ Technol, Dept Elect & Elect Informat Engn, 1-1 Hibarigaoka Tempaku Cho, Toyohashi, Aichi 4418580, Japan

[2] Toyohashi Univ Technol, Dept Environm & Life Sci, Toyohashi, Aichi, Japan

[3] Toyohashi Univ Technol, Elect Inspired Interdisciplinary Res Inst EIIRIS, Toyohashi, Aichi, Japan

[4] Toyohashi Univ Technol, Dept Comp & Sci & Engn, Toyohashi, Aichi, Japan

关键词: Nanowire;    Nanoelectrode;    In vivo;    Intracellular recording;    Extracellular recording;   

DOI  :  10.1016/j.snb.2017.11.152

来源: Elsevier

【 摘 要 】

Nanowire and nanotube-based nanoscale needle devices are powerful tools for intracellular applications, including electrical intracellular recording. However, the applications of these devices to thick biological tissues, such as brain slices or brain tissue in vivo, are limited due to the short length of conventional needles (<10 mu m). In this study, we fabricated 200 mu m- and 400 mu m-long nanoscale-tipped microneedle-electrode (NTE) devices for in vivo applications. The fabricated NTEs penetrated tissue and could be used for both intracellular and extracellular recording, as demonstrated in mouse brain in vivo. Local field potentials (extracellular potentials) were recorded via the 200 mu m-long device, whereas resting membrane potentials (intracellular potentials) were observed via the 400 mu m-long device. Moreover, the NTE device could penetrate the dura mater. Thus, our long NTE device expands the application of nanodevices to intra- and extracellular recordings in vivo, which cannot be achieved using previously available nanoneedle devices, and should therefore contribute to a greater understanding of the nervous system. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

Free