Conditioned taste aversion dependent regulation of amygdala gene expression

PHYSIOLOGY & BEHAVIOR

卷:105

Article

Panguluri, Siva K.¹  Kuwabara, Nobuyuki¹  Kang, Yi²  Cooper, Nigel¹  Lundy, Robert F.¹ 

[1] Univ Louisville, Sch Med, Dept Anat Sci & Neurobiol, Louisville, KY 40292 USA

[2] St Louis Univ, Sch Med, Dept Pharmacol & Physiol Sci, St Louis, MO 63104 USA

关键词: Learning;    Memory;    Taste aversion;    Insulin;    Amygdala;   

DOI  :  10.1016/j.physbeh.2011.11.001

来源: Elsevier

【 摘 要 】

The present experiments investigated gene expression in the amygdala following contingent taste/LiCl treatment that supports development of conditioned taste aversion (CTA). The use of whole genome chips and stringent data set filtering led to the identification of 168 genes regulated by CIA compared to non- contingent LiCl treatment that does not support CIA learning. Seventy-six of these genes were eligible for network analysis. Such analysis identified behavior as the top biological function, which was represented by 15 of the 76 genes. These genes included several neuropeptides, G protein-coupled receptors, ion channels, kinases, and phosphatases. Subsequent qRT-PCR analyses confirmed changes in mRNA expression for 5 of 7 selected genes. We were able to demonstrate directionally consistent changes in protein level for 3 of these genes; insulin 1, oxytocin, and major histocompatibility complex class I-C. Behavioral analyses demonstrated that blockade of central insulin receptors produced a weaker CTA that was less resistant to extinction. Together, these results support the notion that we have identified downstream genes in the amygdala that contribute to CIA learning. (C) 2011 Elsevier Inc. All rights reserved.

【 授权许可】

Free