Ability of GLP-1 to decrease food intake is dependent on nutritional status

PHYSIOLOGY & BEHAVIOR

卷:135

Article

Ronveaux, Charlotte C.^1,2  de Lartigue, Guillaume¹  Raybould, Helen E.¹ 

[1] Univ Calif Davis, Sch Vet Med, Dept Anat Physiol & Cell Biol, Davis, CA 95616 USA

[2] AgroParisTech, Nutr Physiol & Ingest Behav UMR914, F-75005 Paris, France

关键词: Glucagon-like-peptide-1;    Vagal afferent neurons;    Food intake;    Receptor trafficking;   

DOI  :  10.1016/j.physbeh.2014.06.015

来源: Elsevier

【 摘 要 】

Gut-derived glucagon like peptide-1 (GLP-1) acts in the postprandial period to stimulate insulin secretion and inhibit gastrointestinal motor and secretory function; whether endogenous peripheral GLP-1 inhibits food intake is less clear. We hypothesized that GLP-1 inhibits food intake in the fed, but not fasted, state. There is evidence that GLP-1 acts via stimulation of vagal afferent neurons (VAN); we further hypothesized that the satiating effects of endogenous GLP-1 in the postprandial period is determined either by a change in GLP-1 receptor (GLP-1R) expression or localization to different cellular compartments in VAN. Methods: Food intake was recorded following administration of GLP-1 (50 mu g/kg or 100 mu g/Icg) or saline (IP) in Wistar rats fasted for 18 h or fasted then re-fed with 3 g chow. GLP-1R protein expression and localization on VAN were determined by immunocytochemistry and immunoblots in animals fasted for 18 h or fasted then re-fed for 40 min. GLP-1R mRNA level was detected in animals fasted for 18 h.or fasted and re-fed ad libitum for 2 h. Results: GLP-1 (100 mu g/kg) significantly reduced 40 min food intake by 38% in re-fed but not fasted rats (p < 0.05). GLP-1R mRNA or protein levels in VAN were unchanged in re-fed compared to fasted rats. However, GLP-1R localization to the plasma membrane was significantly increased in VAN by feeding. Conclusion: Feeding changes the ability of peripheral GLP-1 to inhibit food intake. GLP-1Rs are trafficked to the plasma membrane in response to a meal. GLP-1 may play a role in regulating food intake in the postprandial period. (C) 2014 Elsevier Inc All rights reserved.

【 授权许可】

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