Selective determination of poly(styrene) and polyolefin microplastics in sandy beach sediments by gel permeation chromatography coupled with fluorescence detection

MARINE POLLUTION BULLETIN

卷:136

Article

Biver, Tarita¹  Bianchi, Sabrina¹  Carosi, Maria Rita¹  Ceccarini, Alessio¹  Corti, Andrea¹  Manco, Enrico¹  Castelvetro, Valter¹ 

[1] Univ Pisa, Dept Chem & Ind Chem, Via G Moruzzi 13, I-56124 Pisa, Italy

关键词: Gel permeation chromatography;    Fluorescence;    Marine litter;    Microplastics;    Polystyrene;    Oxidized polyolefins;   

DOI  :  10.1016/j.marpolbul.2018.09.024

来源: Elsevier

【 摘 要 】

Microplastics generated by plastics waste degradation are ubiquitous in marine and freshwater basins, posing serious environmental concerns. Raman and FTIR spectroscopies, along with techniques such as pyrolysis-GC/MS, are typically used for their identification. We present a procedure based on gel permeation chromatography (GPC) coupled with fluorescence detection for semi-quantitative selective determination of the most common microplastics found in marine shoreline sediments: poly(styrene) (PS) and partially degraded polyolefins (LDPEox). By operating the detector at either 260/280 or 370/420 nm excitation/emission wavelengths PS can be distinguished from LDPEox upon GPC separation. Semi-quantitative determination of microplastics contents is also possible: dichloromethane extracts of PS and LDPEox yield linear plots of fluorescence peak area vs concentration (0-5.0 mg/mL range) and were used as reference materials for quantification of the microplastics content in sand samples collected in the winter berm and dune sectors of a Tuscany beach in Italy.

【 授权许可】

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