Microbial community and metagenome dynamics during biodegradation of dispersed oil reveals potential key-players in cold Norwegian seawater

MARINE POLLUTION BULLETIN

卷:129

Article

Ribicic, Deni¹  Netzer, Roman²  Hazen, Terry C.³  Techtmann, Stephen M.⁴  Drablos, Finn¹  Brakstad, Odd Gunnar² 

[1] NTNU Norwegian Univ Sci & Technol, Dept Clin & Mol Med, Trondheim, Norway

[2] SINTEF OCEAN, Trondheim, Norway

[3] Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN USA

[4] Michigan Technol Univ, Dept Biol Sci, Houghton, MI 49931 USA

关键词: Biodegradation;    Bioremediation;    Chemical dispersant;    Petroleum;    Metagenomics;    16S rRNA;   

DOI  :  10.1016/j.marpolbul.2018.02.034

来源: Elsevier

【 摘 要 】

Oil biodegradation as a weathering process has been extensively investigated over the years, especially after the Deepwater Horizon blowout. In this study, we performed microcosm experiments at 5 degrees C with chemically dispersed oil in non-amended seawater. We link biodegradation processes with microbial community and metagenome dynamics and explain the succession based on substrate specialization. Reconstructed genomes and 16S rRNA gene analysis revealed that Bermanella and Zhongshania were the main contributors to initial n-alkane breakdown, while subsequent abundances of Colweilia and microorganisms closely related to Porticoccaceae were involved in secondary n-alkane breakdown and beta-oxidation. Cycloclasticus, Porticoccaceae and Sponsiiabcteraceae were associated with degradation of mono- and poly-cyclic aromatics. Successional pattern of genes coding for hydrocarbon degrading enzymes at metagenome level, and reconstructed genomic content, revealed a high differentiation of bacteria involved in hydrocarbon biodegradation. A cooperation among oil degrading microorganisms is thus needed for the complete substrate transformation.

【 授权许可】

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