Paralytic shellfish toxin production by the dinoflagellate Alexandrium pacificum (Chinhae Bay, Korea) in axenic, nutrient-limited chemostat cultures and nutrient-enriched batch cultures

MARINE POLLUTION BULLETIN

卷:104

Article

Han, Myungsoo¹  Lee, Haeok¹  Anderson, Donald M.²  Kim, Baikho¹ 

[1] Hanyang Univ, Dept Life Sci, Seoul 04763, South Korea

[2] Woods Hole Oceanog Inst, Dept Biol, Woods Hole, MA 02543 USA

关键词: Alexandrium pacificum;    Batch cultures;    Chemostat;    Dinoflagellate;    Nutrient enrichment and limitation;    PSP toxin;   

DOI  :  10.1016/j.marpolbul.2016.01.057

来源: Elsevier

【 摘 要 】

Blooms of Alexandrium pacificum (formerly Alexandrium tamarense) are common in Chinhae Bay (Korea), presumably linked to anthropogenic eutrophication. Here we examine PSP toxin content and composition in axenic chemostat and batch cultures of A. pacificum using growth conditions that differed according to dilution rate, nutrient limitations, and enrichments. Phosphate (P)-limited cells in chemostat cultures had higher toxin content and a toxin composition that differed from that of nitrogen (N)-limited cells at the highest growth rates. Therefore, toxin composition changes do occur in axenic cultures of A. pacificum following extended growth under steady state conditions. In nutrient -limited batch cultures that received N and P enrichment, the N-enriched cultures showed a more diverse toxin profile than the P-enriched cells; the toxin content of N-enriched cells was lower than in the P-enriched cultures. We infer the following order for the biosynthesis of individual toxins: C1, C2 > GTX3 > GTX1 > neoSTX. (C) 2016 Elsevier Ltd. All rights reserved.

【 授权许可】

Free