Acute exposure to TiO2 nanoparticles produces minimal apparent effects on oyster, Crassostrea virginica (Gmelin), hemocytes

MARINE POLLUTION BULLETIN

卷:127

Article

Doyle, John J.¹  Ward, J. Evan²  Wikfors, Gary H.³ 

[1] Gloucester Marine Genom Inst, Blackburn Ctr 55, Gloucester, MA 01930 USA

[2] Univ Connecticut, Dept Marine Sci, 1080 Shennecossett Rd, Groton, CT 06340 USA

[3] NOAA, Northeast Fisheries Sci Ctr, 212 Rogers Ave, Milford, CT 06460 USA

关键词: Oyster;    Titania;    Nanoparticles;    Hemocytes;    Photoactivation;    ROS;   

DOI  :  10.1016/j.marpolbul.2017.12.039

来源: Elsevier

【 摘 要 】

The response of oyster (Crassostrea virginica) hernocytes was studied following exposure to anatase nanoparticles (ca. 7.4 nm), surface-coated rutile nanocomposites (UV-Titan M212, ca. 86 nm) and bulk titanium dioxide (TiO2) particles (anatase and rutile crystalline forms; 0.4-0.5 mu m). Hemocytes were collected from oysters and exposed to one of the four particle types at concentrations of 0.1, 0.5, and 1.0 mg/L under dark and environmentally-relevant light conditions for periods of two and four hours. Hemocyte mortality, phagocytosis, and reactive oxygen species (ROS) production were then evaluated using flow-cytometric assays. Bulk and nanoparticulate TiO2 had little effect on viability of oyster hemocytes or on production of ROS. Significant changes in phagocytosis occurred after exposure to anatase nanoparticles for 4 h under dark conditions, and UV-Titan for 2 h under light conditions. Results demonstrate that TiO2 particles (bulk or nanoscale) produce minimal effects on hemocyte biomarkers examined following acute, in vitro exposures.

【 授权许可】

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