Next-generation sequencing and culture-based techniques offer complementary insights into fungi and prokaryotes in beach sands

MARINE POLLUTION BULLETIN

卷:119

Article

Romao, Daniela¹  Staley, Christopher²  Ferreira, Filipa¹  Rodrigues, Raquel¹  Sabino, Raquel³  Verissimo, Cristina³  Wang, Ping²  Sadowsky, Michael^2,4  Brandao, Joao¹ 

[1] Natl Inst Hlth Dr Ricardo Jorge, Dept Environm Hlth, Water & Soils Unit, Av Padre Cruz, P-1649016 Lisbon, Portugal

[2] Univ Minnesota, BioTechnol Inst, St Paul, MN 55108 USA

[3] Natl Inst Hlth Dr Ricardo Jorge, Dept Infect Dis, Reference Unit Parasit & Fungal Infect, Av Padre Cruz, P-1649016 Lisbon, Portugal

[4] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA

关键词: Beach sand;    Fungal community structure;    Monitoring;    Prokaryotic community structure;    Public health;   

DOI  :  10.1016/j.marpolbul.2017.04.036

来源: Elsevier

【 摘 要 】

A next-generation sequencing (NGS) approach, in conjunction with culture-based methods, was used to examine fungal and prokaryotic communities for the presence of potential pathogens in beach sands throughout Portugal. Culture-based fungal enumeration revealed low and variable concentrations of the species targeted (yeasts and dermatophytes), which were underrepresented in the community characterized by NGS targeting the ITS1 region. Conversely, NGS indicated that the potentially pathogenic species Purpureocillium liliacinum comprised nearly the entire fungal community. Culturable fecal indicator bacterial concentrations were low throughout the study and unrelated to communities characterized by NGS. Notably, the prokaryotic communities characterized revealed a considerable abundance of archaea. Results highlight differences in communities between methods in beach sand monitoring but indicate the techniques offer complementary insights. Thus, there is a need to leverage culture-based methods with NGS methods, using a toolbox approach, to determine appropriate targets and metrics for beach sand monitoring to adequately protect public health.

【 授权许可】

Free