Overall bacterial community composition and abundance of nitrifiers and denitrifiers in a typical macrotidal estuary

MARINE POLLUTION BULLETIN

卷:126

Article

Zhu, Weijing¹  Wang, Cheng¹  Sun, Faqian¹  Zhao, Liancheng²  Dou, Wenjie²  Mao, Zhihua²  Wu, Weixiang¹ 

[1] Zhejiang Univ, Inst Environm Sci & Technol, Zhejiang Prov Key Lab Water Pollut Control & Envi, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China

[2] State Ocean Adm, Inst Oceanog 2, State Key Lab Satellite Ocean Environm Dynam, 36 Baochu North Rd, Hangzhou 310012, Zhejiang, Peoples R China

关键词: Nitrogen;    AOB amoA;    nirS;    Bacterial communities;    Estuary;    Sediment;   

DOI  :  10.1016/j.marpolbul.2017.09.062

来源: Elsevier

【 摘 要 】

Coupled nitrogen cycling processes can alleviate the negative effects of eutrophication caused by excessive nitrogen load in estuarine ecosystems. The abundance and diversity of nitrifiers and denitrifiers across different environmental gradients were examined in the sediment of Hangzhou Bay. Quantitative PCR and Pearson's correlation analyses suggested that the bacterial ammonia-oxidizers (AOB) were the dominant phylotypes capable of ammonia oxidation, while the nirS-encoding denitrifiers predominated in the denitrification process. Simultaneously, nitrite and pH were found to be the two major factors influencing amoA and nir gene abundances, and the distribution of bacterial communities. Moreover, the ratio of nirS/AOB amoA gene abundance showed negative correlation with nitrite concentration. Fluorescence in situ hybridization further demonstrated that AOB and acetate-denitrifying cells were closely connected and formed obvious aggregates in the sediment. Together, all these results provided us a preliminary insight for coupled nitrification-denitrification processes in the sediment of Hangzhou Bay.

【 授权许可】

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