Detection and characterisation of the biopollutant Xenostrobus securis (Lamarck 1819) Asturian population from DNA Barcoding and eBarcoding

MARINE POLLUTION BULLETIN

卷:105

Article

Devloo-Delva, Floriaan¹  Miralles, Laura¹  Ardura, Alba²  Sorrell, Yaisel J.¹  Pejovic, Ivana¹  Tsartsianidou, Valentina¹  Garcia-Vazquez, Eva¹ 

[1] Univ Oviedo, Dept Funct Biol, C Julian Claveria S-N, Oviedo 33006, Spain

[2] Univ Perpignan, CRIOBE CNRS EPHE USR3278, Lab Excellence CORAIL, CBETM, 58 Rue Paul Alduy, F-66860 Perpignan, France

关键词: Xenostrobus securis;    biological invasion;    environmental DNA;    Barcoding;    phylogenetic expansion signals;    Bay of Biscay;   

DOI  :  10.1016/j.marpolbul.2016.03.008

来源: Elsevier

【 摘 要 】

DNA efficiently contributes to detect and understand marine invasions. In 2014 the potential biological pollutant pygmy mussel (Xenostrobus securis) was observed for the first time in the Aviles estuary (Asturias, Bay of Biscay). The goal of this study was to assess the stage of invasion, based on demographic and genetic (DNA Barcoding) characteristics, and to develop a molecular tool for surveying the species in environmental DNA. A total of 130 individuals were analysed for the DNA Barcode cytochrome oxidase I gene in order to determine genetic diversity, population structure, expansion trends, and to inferring introduction hits. Reproduction was evidenced by bimodal size distributions of 1597 mussels. High population genetic variation and genetically distinct clades might suggest multiple introductions from several source populations. Finally, species-specific primers were developed within the DNA barcode for PCR amplification from water samples in order to enabling rapid detection of the species in initial expansion stages. (C) 2016 Elsevier Ltd. All rights reserved.

【 授权许可】

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