Development of a fast and efficient method to analyze microplastics in planktonic samples

MARINE POLLUTION BULLETIN

卷:168

Article

Lopez-Rosales, Adrian¹  Andrade, Jose M.¹  Grueiro-Noche, Gloria¹  Fernandez-Gonzalez, Veronica¹  Lopez-Mahia, Purificacion¹  Muniategui-Lorenzo, Soledad¹ 

[1] Univ A Coruna, Inst Environm Chem IUMA, Grp Appl Analyt Chem, Campus Zapateira S-N, La Coruna 15071, Spain

关键词: Plankton;    Microplastics;    Infrared microscopy;    Oxidative digestion;    Alkaline digestion;    Enzymatic digestion;   

DOI  :  10.1016/j.marpolbul.2021.112379

来源: Elsevier

【 摘 要 】

Microplastics (MPs) affect plankton (a basis of the trophic chain) and planktivorous fish can ingest them through food confusion or by trophic transmission. Consensus to determine MPs in plankton is lacking and, so, three digestion treatments were evaluated: Alkaline (potassium hydroxide) and enzymatic (protease plus lipase) digestions, both combined with a hydrogen peroxide stage; and an oxidative method using a surfactant (sodium dodecyl sulfate) plus hydrogen peroxide. The alkaline method using potassium hydroxide was found to damage polystyrene. MPs were identified with a stereomicroscope and characterized by reflectance infrared microscopy in semi-automatic mode (using dedicated multi-well aluminium plates). Analytical recoveries for polypropylene, polystyrene, polyethylene, polyamide, polyvinyl chloride and polyethylene terephthalate were higher than 75%, 82% and 83% for the alkaline, enzymatic and oxidative treatments, respectively. The enzymatic method was successfully validated in a European interlaboratory exercise and the oxidative method was demonstrated to be a reliable, fast and cheaper alternative.

【 授权许可】

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