The serotonergic hallucinogen 5-methoxy-N,N-dimethyltryptamine disrupts cortical activity in a regionally-selective manner via 5-HT1A and 5-HT2A receptors

NEUROPHARMACOLOGY

卷:101

Article

Riga, Maurizio S.^1,2,3  Bortolozzi, Analia^1,2,3  Campa, Letizia^1,2,3  Artigas, Francesc^1,2,3  Celada, Pau^1,2,3 

[1] Inst Invest Biomed August Pi & Sunyer IDIBAPS, Barcelona, Spain

[2] CSIC, IDIBAPS, Inst Invest Biomed Barcelona, Dept Neurochem & Neuropharmacol, Barcelona, Spain

[3] Ctr Invest Biomed Red Salud Mental CIBERSAM, Madrid, Spain

关键词: Prefrontal cortex;    Sensorial cortical areas;    Visual cortex;    Hallucinogens;    5-HT receptors;    Oscillatory activity;   

DOI  :  10.1016/j.neuropharm.2015.10.016

来源: Elsevier

【 摘 要 】

5-Methoxy-N,N-dimethyltryptamine (5-MeO-DMT) is a natural hallucinogen, acting as a non-selective serotonin 5-HT1A/5-HT2A-R agonist. Psychotomimetic agents such as the non-competitive NMDA-R antagonist phencyclidine and serotonergic hallucinogens (DOI and 5-MeO-DMT) disrupt cortical synchrony in the low frequency range (<4 Hz) in rat prefrontal cortex (PFC), an effect reversed by antipsychotic drugs. Here we extend these observations by examining the effect of 5-MeO-DMT on low frequency cortical oscillations (LFCO, <4 Hz) in PFC, visual (V1), somatosensory (Si) and auditory (Au1) cortices, as well as the dependence of these effects on 5-HT1A-R and 5-HT2A-R, using wild type (WT) and 5-HT2A-R knockout (KO2A) anesthetized mice. 5-MeO-DMT reduced LFCO in the PFC of WT and KO2A mice. The effect in KO2A mice was fully prevented by the 5-HT1A-R antagonist WAY-100635. Systemic and local 5-MeO-DMT reduced 5-HT release in PFC mainly via 54-IT1A-R. Moreover, 5-MeO-DMT reduced LFCO in Si, Au1 and V1 of WT mice and only in V1 of KO2A mice, suggesting the involvement of 5-HT1A-R activation in the 5-MeO-DMT-induced disruption of V1 activity. In addition, antipsychotic drugs reversed 5-MeO-DMT effects in WT mice. The present results suggest that the hallucinogen action of 5-MeO-DMT is mediated by simultaneous alterations of the activity of sensory (Si, Au1, V1) and associative (PFC) cortical areas, also supporting a role of 5-HT1A-R stimulation in V1 and PFC, in addition to the well-known action on 5-HT2A-R. Moreover, the reversal by antipsychotic drugs of 5-MeO-DMT effects adds to previous literature supporting the usefulness of the present model in antipsychotic drug development. (C) 2015 Elsevier Ltd. All rights reserved.

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