Type-1 cannabinoid receptors colocalize with caveolin-1 in neuronal cells

NEUROPHARMACOLOGY

卷:54

Article

Bari, Monica²  Oddi, Sergio^1,3  De Simone, Chiara^2,3  Spagnolo, Paola²  Gasperi, Valeria^1,3  Battista, Natalia¹  Centonze, Diego^3,4  Maccarrone, Mauro^1,3 

[1] Univ Teramo, Dept Biomed Sci, I-64100 Teramo, Italy

[2] Univ Roma Tor Vergata, Dept Expt Med & Biochem Sci, I-00133 Rome, Italy

[3] S Lucia Fdn, IRCCS, CERC, European Ctr Brain Res, I-00196 Rome, Italy

[4] Univ Roma Tor Vergata, Dept Neurosci, Neurol Clin, I-00133 Rome, Italy

关键词: caveolin;    cholesterol;    endocannabinoids;    G protein-coupled receptors;    lipid rafts;    neurodegeneration;    signal transduction;    trafficking;   

DOI  :  10.1016/j.neuropharm.2007.06.030

来源: Elsevier

【 摘 要 】

Type-1 (CB1) and type-2 (CB2) cannabinoid receptors belong to the rhodopsin family of G protein-coupled receptors, and are activated by endogenous lipids termed endocannabinoids. Recent reports have demonstrated that CB1R, unlike CB2R and other receptors and metabolic enzymes of endocannabinoids, functions in the context of lipid rafts, i.e. plasma membrane microdomains which may be important in modulating signal transduction. Here, we present novel data based on cell subfractionation, immunoprecipitation and confocal microscopy studies, that show that in C6 cells CB1R co-localizes almost entirely with caveolin-1. We also show that trafficking of CB1R in response to the raft disruptor methyl-beta-cyclodextrin (MCD) is superimposable on that of caveolin-1, and that MCD treatment increases the accessibility of CB1R to its specific antibodies. These findings may be relevant for the manifold CB1R-dependent activities of endocannabinoids, like the regulation of apoptosis and of neurodegenerative diseases. (c) 2007 Elsevier Ltd. All rights reserved.

【 授权许可】

Free