Noradrenergic antidepressant responses to desipramine in vivo are reciprocally regulated by arrestin3 and spinophilin

NEUROPHARMACOLOGY

卷:62

Article

Cottingham, Christopher¹  Li, Xiaohua²  Wang, Qin¹ 

[1] Univ Alabama Birmingham, Dept Physiol & Biophys, Birmingham, AL 35294 USA

[2] Univ Alabama Birmingham, Dept Psychiat & Behav Neurobiol, Birmingham, AL 35294 USA

关键词: alpha(2) Adrenergic receptor;    Arrestin;    Depression;    Desipramine;    Forced swim test;    Spinophilin;   

DOI  :  10.1016/j.neuropharm.2012.02.011

来源: Elsevier

【 摘 要 】

Many antidepressant drugs, including the tricyclic antidepressant desipramine (DMI), are broadly understood to function by modulating central noradrenergic neurotransmission. alpha(2) adrenergic receptors (alpha(2)ARs) are key regulators of the noradrenergic system, and previous work has implicated alpha(2)ARs in mediating the antidepressant activity of DMI in the rodent forced swim test (FST). However, little is known about intracellular regulators of antidepressant drug action. alpha(2)AR function is tightly regulated by its intracellular interacting partners arrestin and the dendritic protein spinophilin. We have previously established the competitive and reciprocal nature of these interacting proteins at the alpha(2)AR in the context of classic agonist effects, and have shown DMI to be a direct arrestin-biased ligand at the receptor. In the present study, we report that mice deficient in the alpha(2A)AR subtype lack DMI-induced antidepressant behavioral effects in the FST. As well, mice deficient in arrestin3 lack antidepressant response to DMI, while spinophilin-null mice have enhanced antidepressant response to DMI compared with wild-type controls, indicating that this alpha(2A)AR-mediated response is reciprocally regulated by arrestin and spinophilin. The characteristic of alpha(2A)AR-dependence and arrestin3 involvement was shared by the antidepressant effect of the classic alpha(2)AR agonist clonidine but not the non-tricyclic norepinephrine reuptake inhibitor reboxetine, supporting a model whereby DMI exerts its antidepressant effect through direct engagement of the alpha(2A)AR and arrestin3. Our results implicate arrestin- and spinophilin-mediated regulation of the alpha(2A)AR in the pharmacology of the noradrenergic antidepressant DMI, and suggest that manipulation of this mode of receptor regulation may represent a novel and viable therapeutic strategy. (C) 2012 Elsevier Ltd. All rights reserved.

【 授权许可】

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